Anti-2,2,7-trimethylguanosine (m₃G/TMG) mAb

Availability (in Japan)

10 or more

（In Japan at 00:05,
Dec 5, 2024 in JST）

Size

200 µL (1 mg/mL)

Data Sheet

SDS

Data	Immunoprecipitation Immunocytochemistry
Clonality	Monoclonal	Clone	235-1
Isotype (Immunized Animal)	Mouse IgG2aκ
Applications	IP 10 µg/sample IC 0.5-1 µg/mL
Immunogen (Antigen)	Carrier protein-conjugated trimethylguanosine (m₃G/TMG)
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	N2,N2,7-Trimethylguanosine, m2,2,7G
Background	Small nuclear RNA (snRNA) is known to be involved in pre-mRNA splicing as an RNA component of snRNP. 2,2,7-trimethylated guanosine (m₃G/TMG) is a modified nucleoside at the 5’-cap site of U snRNAs except for U6 snRNA. Shortly after transcription, 7-methylguanosine (m⁷G), called cap structure, is added to 5’-end of newly synthesized U snRNA and m⁷G-capped U snRNA is exported to the cytoplasm. The m⁷G-cap is converted to m₃G-cap, which is a hypermethylated structure of 5’-end, by cytoplasmic catalytic protein TSG1 and the m₃G-capped U snRNA forms snRNP with Sm proteins in the cytoplasm, followed by entry into the nucleus. The m₃G-capped snRNP plays a role in regulation of pre-mRNA splicing as a part of spliceosome in nucleus. It is also reported that U3 and U8 snoRNA, localized to Cajal body during RNP biogenesis, possess m₃G-cap structure.
Related products	RN1005 RIP-Assay Kit for microRNA D347-3 Anti-Pseudouridine mAb D345-3 Anti-1-methyladenosine (m¹A) mAb D346-3 Anti-5-methylcytidine (m⁵C) mAb PM077 Anti-5-hydroxymethylcytosine (5hmC) pAb MI-11-3 Anti-Bromodeoxyuridine mAb MI-11-5 Anti-Bromodeoxyuridine mAb-PE RN095PW Anti-SNRNP200 pAb M218-3 Anti-5-hydroxymethylcytosine (5hmC) mAb M227-3 Anti-Digoxigenin (DIG) mAb M228-3 Anti-FITC mAb RN077PW Anti-SMN1 pAb RN124PW Anti-RNMT (Human) pAb RN016M Anti-7-methylguanosine (m⁷G)-Cap mAb RN017M Anti-7-methylguanosine (m⁷G) mAb PM098 Anti-Inosine pAb
References	Nottrott S et al. Functional interaction of a novel 15.5kD [U4/U6.U5] tri-snRNP protein with the 5' stem-loop of U4 snRNA. EMBO J. 18, 6119-33 (1999)（PMID: 10545122） Nagai K et al. Structure and assembly of the spliceosomal snRNPs. Novartis Medal Lecture. Biochem Soc Trans. 29, 15-26 (2001)（PMID: 11356120） Fox AH et al. Paraspeckles: a novel nuclear domain. Curr Biol. 12, 13-25 (2002)（PMID: 11790299） Yedavalli VS, Jeang KT. Trimethylguanosine capping selectively promotes expression of Rev-dependent HIV-1 RNAs. PNAS 107, 14787-92 (2010)（PMID: 20679221） Tsuiji H et al. Spliceosome integrity is defective in the motor neuron diseases ALS and SMA. EMBO Mol. Med. 5, 221-34 (2013)（PMID: 23255347）
Product category	Research area RNA-RNP Network Epigenetics Brands RiboCluster Profiler™

The availability is based on the information in Japan at 00:05, Dec 5, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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