Anti-7-methylguanosine (m⁷G)-Cap mAb

Availability (in Japan)

10 or more

（In Japan at 17:00,
Oct 8, 2024 in JST）

Size

200 µL (1 mg/mL)

Data Sheet

SDS

This antibody (Clone 150-15) reacts with 5′-terminal 7-methylguanosine (m⁷G) cap structure of RNA and partially cross-reacts with m⁷G within RNA.

Data	Immunoprecipitation Immunoprecipitation Dot Blotting
Clonality	Monoclonal	Clone	150-15
Isotype (Immunized Animal)	Mouse IgG2aκ
Applications	IP 10 µg/sample IC Can be used. ELISA Can be used. DB 1 µg/mL
Immunogen (Antigen)	Carrier protein-conjugated 7-methylguanosine (m⁷G)-Cap analogue
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	5’-cap, 7mGppp, m7G(5′)ppp(5′)X
Background	The 7-methylguanosine (m⁷G) cap, also known as m7G(5′)ppp(5′)X, is a specific structure located on the 5′-terminus of the eukaryotic mRNA and in certain viral RNAs. This cap structure is also found at the 5′-terminus of mRNA-like lncRNA and pri-miRNA. The m⁷G cap is primarily involved in the translation of mRNAs. It is also responsible for the stability of the mRNA, and promotes transcription, splicing, polyadenylation, and nuclear export of the mRNA.
Related products	RN017M Anti-7-methylguanosine (m⁷G) mAb RN1005 RIP-Assay Kit for microRNA D347-3 Anti-Pseudouridine mAb D345-3 Anti-1-methyladenosine (m¹A) mAb D346-3 Anti-5-methylcytidine (m⁵C) mAb PM077 Anti-5-hydroxymethylcytosine (5hmC) pAb MI-11-3 Anti-Bromodeoxyuridine mAb MI-11-5 Anti-Bromodeoxyuridine mAb-PE M218-3 Anti-5-hydroxymethylcytosine (5hmC) mAb M227-3 Anti-Digoxigenin (DIG) mAb M228-3 Anti-FITC mAb RN019M Anti-2,2,7-trimethylguanosine (m₃G/TMG) mAb RN006M Anti-EIF4E mAb RN001P Anti-EIF4E pAb RN126PW Anti-AHCY (SAHH) pAb RN124PW Anti-RNMT (Human) pAb PM098 Anti-Inosine pAb
References	Rottman F et al. Sequences containing methylated nucleotides at the 5' termini of messenger RNAs: possible implications for processing. Cell 3, 197-9 (1974)（PMID: 4373171 ） Cowling VH. Regulation of mRNA cap methylation. Biochem J. 425, 295-302 (2009)（PMID: 20025612 ） Ramanathan A et al. mRNA capping: biological functions and applications. Nucleic Acids Res. (2016) In press.（PMID: 27317694 ）
Product category	Research area RNA-RNP Network Epigenetics Brands RiboCluster Profiler™

The availability is based on the information in Japan at 17:00, Oct 8, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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