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HOME > Product search results > Code No. RN009P Anti-IGF2BP3 (IMP3) pAb

Code No.　RN009P

Anti-IGF2BP3 (IMP3) pAb

Availability (in Japan)

10 or more

（In Japan at 17:00,
Apr 16, 2024 in JST）

Size

200 µL (1 mg/mL)

Data

  • Western Blotting

  • Immunoprecipitation

  • RNP Immunoprecipitation
Clonality Polyclonal Clone Polyclonal
Isotype (Immunized Animal) Rabbit Ig (aff.)
Applications
WB
1:1,000  
IP
5 µg/250 µL of cell extract from 2.5 x 106 cells  
IC*
  （PMID: 26990993 / 27068461）
RIP
15 µg/500 µL of cell extract from 1.2 x 107 cells  
Other(eCLIP)
reported.  （PMID: 27068461 / 27108577）
Other(iCLIP)
reported.  （PMID: 26974154）
Immunogen (Antigen) KLH-conjugated synthetic peptide HQQQKALQSGPPQSRRK (563-579 aa)
Reactivity [Gene ID]

Human[10643], Mouse[140488], Rat(-), Hamster(-)
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names IGF2BP3, insulin-like growth factor 2 mRNA binding protein 3, CT98, KOC1, IMP-3, VICKZ3/IMP3, Koc13, mimp3, Neilsen
Background IGF2BP3/IMP3 is identical to the KH domain containing protein overexpressed in cancer (KOC). The protein encoded by this gene was found to bind in vivo to IGF2 leader 3 and leader 4 mRNAs and H19 RNA. Binding of IMP3 to the 5' UTR of the IGF2 leader 3 mRNA represses translation of IGF2 during late development. The encoded protein contains several KH domains, which are important for RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes. The growth regulating gene IMP3 has been characterized as a candidate for Silver-Russell syndrome.
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Citations

Western Blotting

  1. Ueki A et al. Up-regulation of Imp3 confers in vivo tumorigenicity on murine osteosarcoma cells. PLoS One. 7, e50621 (2012)(PMID:23226335)
  2. Lederer M et al. The role of the oncofetal IGF2 mRNA-binding protein 3 (IGF2BP3) in cancer. Semin Cancer Biol. 29, 3-12 (2014)(PMID:25068994)
  3. Conway AE et al. Enhanced CLIP uncovers IMP protein-RNA ｔargets in ｈuman pluripotent stem cells important for cell adhesion and survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
  4. Degrauwe N et al. The RNA Binding Protein IMP2 Preserves Glioblastoma Stem Cells by Preventing let-7 Target Gene Silencing. Cell Rep 15, 1634-47 (2016)(PMID:27184842)
  5. JnBaptiste CK et al. Dicer loss and recovery induce an oncogenic switch driven by transcriptional activation of the oncofetal Imp1-3 family. Genes Dev. 31, 674-687 (2017)(PMID:28446596)
  6. Hosono Y et al. Oncogenic Role of THOR, a Conserved Cancer/Testis Long Non-coding RNA. Cell. 171, 1559-1572 (2017)(PMID:29373831)

Immunoprecipitation

  1. Conway AE et al. Enhanced CLIP uncovers IMP protein-RNA ｔargets in ｈuman pluripotent stem cells important for cell adhesion and survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
  2. Hosono Y et al. Oncogenic Role of THOR, a Conserved Cancer/Testis Long Non-coding RNA. Cell. 171, 1559-1572 (2017)(PMID:29373831)

Immunocytochemistry

  1. Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993)
  2. Conway AE et al. Enhanced CLIP uncovers IMP protein-RNA ｔargets in ｈuman pluripotent stem cells important for cell adhesion and survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
  3. Markmiller S et al. Context-Dependent and Disease-Specific Diversity in Protein Interactions within Stress Granules. Cell. 172, 590-604.e13 (2018)(PMID:29373831)

RNP Immunoprecipitation

  1. Pasiliao CC et al. The involvement of insulin-like growth factor 2 binding protein 3 (IMP3) in pancreatic cancer cell migration, invasion, and adhesion. BMC Cancer 15, 266 (2015)(PMID:25886367)
  2. Ennajdaoui H et al. IGF2BP3 modulates the Interaction of invasion-associated transcripts with RISC. Cell Rep. 15, 1876-83 (2016)(PMID:27210763)
  3. Kim HY et al. IMP2 and IMP3 cooperate to promote the metastasis of triple-negative breast cancer through destabilization of progesterone receptor. Cancer Lett. 415, 30-39 (2018)(PMID:29217458)

Other(eCLIP)

  1. Conway AE et al. Enhanced CLIP uncovers IMP protein-RNA ｔargets in ｈuman pluripotent stem cells important for cell adhesion and survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
  2. Van Nostrand EL et al. Robust transcriptome-wide discovery of RNA-binding protein binding sites with enhanced CLIP (eCLIP). Nat Methods. 13, 508-14 (2016)(PMID:27108577)

Other(iCLIP)

  1. Palanichamy JK et al. RNA-binding protein IGF2BP3 targeting of oncogenic transcripts promotes hematopoietic progenitor proliferation. J Clin Invest. 126, 1495-511 (2016)(PMID:26974154)
Product category
Research area
RNA-RNP Network
Cancer
Brands
RiboCluster Profiler™
この製品に関するお問い合わせをする

  • The availability is based on the information in Japan at 17:00, Apr 16, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.