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HOME > Product search results > Code No. RN008P Anti-IGF2BP2 (IMP2) pAb

Code No.　RN008P

Anti-IGF2BP2 (IMP2) pAb

Availability (in Japan)

1-9

（In Japan at 00:05,
Apr 19, 2024 in JST）

Size

200 µL (1 mg/mL)

Data

  • Western Blotting

  • Immunoprecipitation

  • RNP Immunoprecipitation
Clonality Polyclonal Clone Polyclonal
Isotype (Immunized Animal) Rabbit Ig (aff.)
Applications
WB
1:1,000  
IP
5 µg/250 µL of cell extract from 2.5 x 106 cells  
IC
Can be used.  
RIP
15 µg/500 µL of cell extract from 1.2 x 107 cells  
Other(eCLIP)
reported.  （PMID: 27018577 / 27068461）
Other(PAR-CLIP)
reported.  （PMID: 27184842）
Immunogen (Antigen) KLH-conjugated synthetic peptide KQQEQKYPQGVASQRSK (583-599 aa)
Reactivity [Gene ID]

Human[10644](PMID: 22899010), Mouse[319765](PMID: 22899010), Rat[303824](PMID: 22513850), Hamster(-)
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names insulin-like growth factor 2 mRNA binding protein 2, p62, IMP-2, VICKZ2/Igf2bp2, Imp2, Neilsen, C330012H03Rik/RGD1305614
Background IGF2BP2 is an RNA binding protein that post-transcriptionally regulates IGF2 during developmental stages. IGF2 mRNA-binding protein family (IGF2BP1, 2 and 3) bind UTR region of IGF2 transcripts and are implicated to in transport of RNA targets to enable protein synthesis at specific locations in the cytoplasm. IGF2BP family members show tissue-selective expression during development. IGF2BP2 is expressed in fetal lung, kidney, thymus, placenta and liver. Genetic valuation in IGF2BP2 was implicated to interact with Fetal Malnutrition on glucose metabolism.
Related products RN1001 RIP-Assay Kit
RN1005 RIP-Assay Kit for microRNA
RN001M Anti-IGF2BP1 (IMP1) mAb
RN007P Anti-IGF2BP1 (IMP1) pAb
RN009P Anti-IGF2BP3 (IMP3) pAb
Citations

Western Blotting

  1. Gong C et al. A long non-coding RNA, LncMyoD, regulates skeletal muscle differentiation by blocking IMP2-mediated mRNA translation. Dev Cell. 34, 181-91 (2015)(PMID:26143994)
  2. Conway AE et al. Enhanced CLIP Uncovers IMP Protein-RNA Targets in Human Pluripotent Stem Cells Important for Cell Adhesion and Survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
  3. Degrauwe N et al. The RNA Binding Protein IMP2 Preserves Glioblastoma Stem Cells by Preventing let-7 Target Gene Silencing. Cell Rep. 15, 1634-47 (2016)(PMID:27184842)
  4. JnBaptiste CK et al. Dicer loss and recovery induce an oncogenic switch driven by transcriptional activation of the oncofetal Imp1-3 family. Genes Dev. 31, 674-687 (2017)(PMID:28446596)
  5. Hosono Y et al. Oncogenic Role of THOR, a Conserved Cancer/Testis Long Non-coding RNA. Cell 171, 1559-1572 (2017)(PMID:29245011)

Immunoprecipitation

  1. Janiszewska M et al. Imp2 controls oxidative phosphorylation and is crucial for preserving glioblastoma cancer stem cells. Genes Dev. 26, 1926-44 (2012)(PMID:22899010)
  2. Conway AE et al. Enhanced CLIP Uncovers IMP Protein-RNA Targets in Human Pluripotent Stem Cells Important for Cell Adhesion and Survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
  3. Degrauwe N et al. The RNA Binding Protein IMP2 Preserves Glioblastoma Stem Cells by Preventing let-7 Target Gene Silencing. Cell Rep. 15, 1634-47 (2016)(PMID:27184842)
  4. Hosono Y et al. Oncogenic Role of THOR, a Conserved Cancer/Testis Long Non-coding RNA. Cell 171, 1559-1572 (2017)(PMID:29245011)

Immunocytochemistry

  1. Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993)
  2. Conway AE et al. Enhanced CLIP Uncovers IMP Protein-RNA Targets in Human Pluripotent Stem Cells Important for Cell Adhesion and Survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
  3. Markmiller S et al.  Context-Dependent and Disease-Specific Diversity in Protein Interactions within Stress Granules. Cell 172, 590-604.e13 (2018)(PMID:29373831)

RNP Immunoprecipitation

  1. Janiszewska M et al. Imp2 controls oxidative phosphorylation and is crucial for preserving glioblastoma cancer stem cells. Genes Dev. 26, 1926-44 (2012)(PMID:22899010)
  2. Schaeffer V et al. RNA-binding protein IGF2BP2/IMP2 is required for laminin-β2 mRNA translation and is modulated by glucose concentration. Am J Physiol Renal Physiol. 303, F75-82 (2012)(PMID:22513850)
  3. Kim HY et al. IMP2 and IMP3 cooperate to promote the metastasis of triple-negative breast cancer through destabilization of progesterone receptor. Cancer Lett. 415, 30-39 (2018)(PMID:29217458)

Other(eCLIP)

  1. Van Nostrand EL et al. Robust transcriptome-wide discovery of RNA-binding protein binding sites with enhanced CLIP (eCLIP). Nat Methods. 13, 508-14 (2016)(PMID:27018577)
  2. Conway AE et al. Enhanced CLIP Uncovers IMP Protein-RNA Targets in Human Pluripotent Stem Cells Important for Cell Adhesion and Survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
  3. Van Nostrand EL et al. CRISPR/Cas9-mediated integration enables TAG-eCLIP of endogenously tagged RNA binding proteins. Methods. 118-119, 50-59 (2017)(PMID:28003131)

Other(PAR-CLIP)

  1. Degrauwe N et al. The RNA Binding Protein IMP2 Preserves Glioblastoma Stem Cells by Preventing let-7 Target Gene Silencing. Cell Rep. 15, 1634-47 (2016)(PMID:27184842)
Product category
Research area
RNA-RNP Network
Brands
RiboCluster Profiler™
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  • The availability is based on the information in Japan at 00:05, Apr 19, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.