Anti-monomeric Azami-Green 1 pAb

Availability (in Japan)

10 or more

（In Japan at 00:05,
Apr 19, 2024 in JST）

Size

100 µL

Citations

Data Sheet

SDS

Data	Western Blotting Immunoprecipitation Immunocytochemistry Immunohistochemistry
Clonality	Polyclonal	Clone	Polyclonal
Isotype (Immunized Animal)	Rabbit Ig (aff.)
Applications	WB 1:1,000 IP 2 µL/sample IC 1:100 IH 1:100 (Heat treatment is necessary.)
Immunogen (Antigen)	Recombinant monomeric Azami-Green 1
Storage buffer	PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Background	CoralHue^® Azami-Green (AG) has been cloned from the stony coral, whose Japanese name is“Azami-Sango”. It absorbs light maximally at 492 nm and emits green light at 505 nm. Wild-type CoralHue^® AG rapidly matures to form a tetrameric complex. CoralHue^® monomeric Azami-Green (mAG1) that maintains the brilliance and pH stability of the parent protein. PM052 is available for immunostaining of “Fucci-S/G₂/M Green” (Fucci; Fluorescent Ubiquitination-based Cell Cycle Indicator). It is possible to use PM052 for Fucci transgenic strain, B6.Cg-Tg(Fucci)504Bsi mice which express Fucci-S/G₂/M Green.
Related products	AM-V9010M pFucci-S/G₂/M Green-Hyg (Expression vector) AM-V9014M pFucci-S/G₂/M Green (Cloning vector) AM-V9016M pFucci-S/G₂/M Green (Expression vector) AM-V9030M pFucci-S/G₂/M Green (N+C)-Hyg (Expression vector) AM-V9034M pFucci-S/G₂/M Green (N+C) (Cloning vector) PM011M Anti-Azami-Green pAb
Citations	Western Blotting Chiu YL et al. Testicular somatic cells in the stony coral Euphyllia ancora express an endogenous green fluorescent protein. Mol Reprod Dev. 86, 798-811 (2019)(PMID:31056825)
References	Sakaue-Sawano A et al. Tracing the silhouette of individual cells in S/G2/M phase with fluorescence. Chem Biol. 15, 1243-8 (2008) (PMID: 19101468) Sakaue-Sawano A et al. Visualizing spatiotemporal dynamics of multicellular cell-cell progression. Cell 132, 487-98 (2008) (PMID: 18267078)
Product category	Tools Fluorescent proteins

The availability is based on the information in Japan at 00:05, Apr 19, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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