Anti-GST-tag pAb

Availability (in Japan)

1-9

（In Japan at 00:05,
Dec 5, 2024 in JST）

Size

100 µL

Citations

Data Sheet

SDS

Data	Immunoprecipitation
Clonality	Polyclonal	Clone	Polyclonal
Isotype (Immunized Animal)	Rabbit IgG
Applications	WB 1:1,000 IP 5 µL/sample
Immunogen (Antigen)	recombinant bacterial glutathione S-transferase, which was purified from pGEX-5X-3 vector using GSH column
Storage buffer	PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Background	Bacterial Glutathione S-transferase (GST) from Schistosoma japonicum is a widely used protein fusion partner, since it provides exhibits little effect on the biological function of the protein of interest. The GST-tag fusion protein expression system allows for efficient coupling to glutathione (GSH) agarose affinity resins and purification by a single step chromatography. This specific antibody for GST-tag fusion protein is useful for monitoring of the fusion protein expression and affinity purification. It does not recognize mammalian GST.
Related products	PM013-7 Anti-GST-tag pAb-HRP-DirecT M071-3 Anti-GST-tag mAb M209-3 Anti-GST-tag mAb
Citations	Western Blotting Zhou J, Blissard GW. Identification of a GP64 subdomain involved in receptor binding by budded virions of the baculovirus Autographica californica multicapsid nucleopolyhedrovirus. J Virol. 82, 4449-60 (2008)(PMID:18287233) Sato M et al. Identification of Fyn as the binding partner for the WASP N-terminal domain in T cells. Int Immunol. 23, 493-502 (2011)(PMID:21705469) Izumi H, Kaneko Y. Trim32 facilitates degradation of MYCN on spindle poles and induces asymmetric cell division in human neuroblastoma cells. Cancer Res. 74, 5620-30 (2014)(PMID:25100564) Hirano T et al. he Fab fragment of anti-IgE Cε2 domain prevents allergic reactions through interacting with IgE-FcεRIα complex on rat mast cells. Sci Rep. 8, 14237(2018)(PMID:30250145) Nakaya H et al. Secretory expression of thyroid hormone receptor using transgenic silkworms and its DNA binding activity. Protein Expr Purif. 176, 105723 (2020)(PMID:32768455)
Product category	Tools Epitope tags

The availability is based on the information in Japan at 00:05, Dec 5, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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