Anti-MitoPLD (Pld6) mAb

Availability (in Japan)

1-9

（In Japan at 17:00,
Apr 18, 2024 in JST）

Size

100 µL (1 mg/mL)

Citations

Data Sheet

SDS

Data	Western Blotting Immunoprecipitation Immunohistochemistry
Clonality	Monoclonal	Clone	26C46-6
Isotype (Immunized Animal)	Mouse IgG2bκ
Applications	WB 2-5 µg/mL IP 3 µg/mL IH 10 µg/mL
Immunogen (Antigen)	KLH conjugated synthetic peptide, EFDPTKYSFFPQKHRGH (corresponding to amino acidresidues 205-221 of mouse MitoPLD (Pld6)
Reactivity [Gene ID]	Human, Mouse[194908], Rat
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	mZuc (Zucchini), Zuc, Gm10, phospholipase D family, member 6
Background	MitoPLD (Pld6), the ortholog of drosophila Zucchini (Zuc), is a member of the phospholipase D superfamily. MitoPLD is strongly expressed in gonads especially in testis, and is located on the outer mitochondrial membrane. It is reported that MitoPLD hydrolyzes cardiolipin to generate phosphatidic acid (PA) at mitochondrial surface, and promotes mitochondrial fusion. PA, generated by MitoPLD, may work as a signaling molecule to regulate the localization and formation of mitochondria and intermitochondrial cement (nuage). It is also reported that the drosophila MitoPLD ortholog, Zuc, is an endoribonuclease essential for primary piRNA biogenesis by processing long non-coding RNA precursors. MitoPLD^-/- mice show meiotic arrest during spermatogenesis, demethylation and derepression of retrotransposons, and defects in primary piRNA biogenesis, indicating that the role of MitoPLD/Zuc in the piRNA pathway is highly conserved among species.
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Citations	Western Blotting Kabayama Y et al. Roles of MIWI, MILI and PLD6 in small RNA regulation in mouse growing oocytes. Nucleic Acids Res. 45, 5387-5398 (2017)(PMID:28115634)
References	Nishimasu H et al. Structure and function of Zucchini endoribonuclease in piRNA biogenesis. Nature. 491, 284-7 (2012)（PMID: 23064230） Gao Q, Frohman MA. Roles for the lipid-signaling enzyme MitoPLD in mitochondrial dynamics, piRNA biogenesis, and spermatogenesis. BMB Rep. 45, 7-13 (2012)（PMID: 22281006） Huang H et al. piRNA-associated germline nuage formation and spermatogenesis require MitoPLD profusogenic mitochondrial-surface lipid signaling. Dev Cell. 20, 376-87 (2011)（PMID: 21397848） Watanabe T et al. MITOPLD is a mitochondrial protein essential for nage formation and piRNA biogenesis in the mouse germline. Dev Cell. 20, 364-75 (2011)（PMID: 21397847） Choi SY et al. A common lipid links Mfn-mediated mitochondrial fusion and SNARE-regulated exocytosis. Nat Cell Biol. 8, 1255-62 (2006)（PMID: 17028579）
Product category	Research area Stem cell research RNA-RNP Network Epigenetics

The availability is based on the information in Japan at 17:00, Apr 18, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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