Anti-LC3 mAb-HRP-DirecT

Availability (in Japan)

10 or more

（In Japan at 17:00,
Apr 19, 2024 in JST）

Size

50 µL

Citations

Data Sheet

SDS

Data	Western Blotting
Clonality	Monoclonal	Clone	8E10
Isotype (Immunized Animal)	Mouse IgG2aκ
Applications	WB 1:1,000
Immunogen (Antigen)	Human LC3 (MAP1LC3B), 1-120 aa (recombinant)
Reactivity [Gene ID]	Human[81631], Mouse[67443], Rat[64862], Hamster[100769810]
Storage buffer	PBS/Preservative/Stabilizer
Storage temp.	4°C	Conjugate	HRP	Manufacturer	MBL
Alternative names	microtubule-associated protein 1 light chain 3 beta, LC3B, ATG8F, MAP1LC3B-a, MAP1A/1BLC3, MAP1LC3B
Background	Macroautophagy mediates the bulk degradation of cytoplasmic components. These components are delivered to lysosomes via autophagosomes. The microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I (18 kDa). A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II (16 kDa). This antibody can detect both forms of LC3.
Related products	8486 Autophagy Watch M186-3 Anti-LC3 mAb PM036 Anti-LC3 pAb M152-3 Anti-LC3 mAb PD014 Anti-LC3 pAb 8485 Autophagy Ab Sampler Set PM036-PN Positive control for anti-LC3 antibody
Notes	Clone 8E10 reacts with LC3B and does not cross-react with LC3A, LC3C, GATE-16, and GABARAP.
Citations	Western Blotting Ohmae S et al. Actin-binding protein coronin 1A controls osteoclastic bone resorption by regulating lysosomal secretion of cathepsin K. Sci Rep. 7, 41710 (2017)(PMID:28300073) Mori H et al. Induction of selective autophagy in cells replicating hepatitis C virus genome. J Gen Virol. 99, 1643-1657 (2018)(PMID:30311874)
References	Jia W et al. Temporal regulation of intracellular organelle homeostasis in T lymphocytes by autophagy. J Immunol. 186, 5313-22 (2011) Tabata K et al. Rubicon and PLEKHM1 negatively regulate the endocytic/autophagic pathway via a novel Rab7-binding domain. Mol Biol Cell 21, 4162-72 (2010) Mookerjee S et al. Posttranslational modification of ataxin-7 at lysine 257 prevents autophagy-mediated turnover of an N-terminal caspase-7 cleavage fragment. J Neurosci. 29, 15134-44 (2009) Saitoh T et al. Loss of the autophagy protein Atg16L1 enhances endotoxin-induced IL-1beta production. Nature 456, 264-8 (2008) Kabeya Y et al. LC3, GABARAP and GATE16 localize to autophagosomal membrane depending on form-II formation. J Cell Sci. 117, 2805-12 (2004) Mizushima N et al. In vivo analysis of autophagy in response to nutrient starvation using transgenic mice expressing a fluorescent autophagosome marker. Mol Biol Cell 15, 1101-11 (2004) Mizushima N et al. Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells. J Cell Biol. 152, 657-68 (2001)
Product category	Research area Autophagy

The availability is based on the information in Japan at 17:00, Apr 19, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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