Data |
Flow Cytometry

Immunocytochemistry

|
Clonality |
Monoclonal
|
Clone |
3G5 |
Isotype
(Immunized Animal)
|
Mouse IgG1 κ
|
Applications |
- IP
- 5 µg/sample
 - FCM
- 0.1-1 µg/mL
- IC
- 1 µg/mL
 - IH*
- reported. (PMID: 29575648 / 29889831)

|
Immunogen
(Antigen) |
RFP
|
Storage buffer |
1 mg/mL in PBS/50% glycerol, pH 7.2
|
Storage temp. |
-20°C
|
Conjugate |
Unlabeled |
Manufacturer |
MBL |
Alternative names |
Red fluorescent protein
|
Background |
Expression vector containing a tag sequence is commonly used to introduce and express a specific gene into a target cell. Red Fluorescent Protein (RFP) fusion protein expression system is preferably used in various laboratories because of an easy monitoring of fusion proteins. This specific antibody for RFP is useful tool for monitoring of the fusion protein expression. |
Related products |
PM005 Anti-RFP pAb PM005-7 Anti-RFP pAb-HRP-DirecT M155-3 Anti-RFP mAb M165-8 Anti-RFP mAb-Agarose M204-3 Anti-RFP mAb M204-7 Anti-RFP mAb HRP-DirecT M208-3 Anti-RFP mAb Cocktail M165-11 Anti-RFP mAb-Magnetic Beads M165-3 Anti-RFP mAb PM073 Anti-Renilla GFP pAb
|
Citations |
Western Blotting - Kobayashi T et al. Cooperation of the IFT-A complex with the IFT-B complex is required for ciliary retrograde protein trafficking and GPCR import. Mol Biol Cell. 32, 45-56 (2020)(PMID:33175651)
Immunoprecipitation - Yamamoto H et al. Functional cross-talk between Rab14 and Rab4 through a dual effector, RUFY1/Rabip4. Mol Biol Cell. 21, 2746-55 (2010)(PMID:20534812)
- Zhao Q et al. Dual Roles of Two Isoforms of Autophagy-related Gene ATG10 in HCV-Subgenomic replicon Mediated Autophagy Flux and Innate Immunity. Sci Rep. 7, 11250 (2017)(PMID:28900156)
Immunocytochemistry - Taguwa S et al. Dysfunction of autophagy participates in vacuole formation and cell death in cells replicating hepatitis C virus. J Virol. 85, 13185-94 (2011)(PMID:21994453)
- Fujimoto I et al. Necdin controls EGFR signaling linked to astrocyte differentiation in primary cortical progenitor cells. Cell Signal. 28, 94-107 (2016)(PMID:26655377)
Immunohistochemistry - Sumiyoshi K et al. Spontaneous development of intratumoral heterogeneity in a transposon-induced mouse model of glioma. Cancer Sci. 109, 1513-1523 (2018)(PMID:29575648)
- Ojima N et al. Genetic dissection of stress-induced reproductive arrest in Drosophila melanogaster females. PLoS Genet. 14, e1007434 (2018)(PMID:29889831)
|
Product category |
- Tools
- Epitope tags
Fluorescent proteins
|