Anti-Caspase-3 (Human) mAb

Availability (in Japan)

1-9

（In Japan at 17:00,
Apr 18, 2024 in JST）

Size

100 µL (1 mg/mL)

Data Sheet

SDS

Data	Western Blotting
Clonality	Monoclonal	Clone	1F9
Isotype (Immunized Animal)	Mouse IgG1
Applications	WB 1 µg/mL IH 10 µg/mL
Immunogen (Antigen)	Recombinant Human caspase-3
Reactivity [Gene ID]	Human[836], Mouse(-), Rat(-)
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	CASP3
Background	Caspase-3 (also known as CPP32, Yama, apopain) is a key member of the caspase family of cysteine proteases. Caspase-3 exists in cells as an inactive 32 kDa proenzyme. During apoptosis procaspase-3 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases, such as caspase-6, -8, or -9. The processed form of caspase-3 consists of large (17 kDa) and small (12 kDa) subunits which associate to form the active tetrameric enzyme tetramer (a pair of heterodimers). The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP, SREBPs, and DFF). Activation of procaspase-3 stands at a point of convergence for the two major types of apoptosis signaling pathways-those linked to cell surface death receptors and those linked to mitochondrial release of cytochrome c.
Related products	M097-3 Anti-Caspase-3 (Human) mAb M059-3 Anti-Caspase-10 (Human) mAb M029-3 Anti-Caspase-4 (Human) mAb M060-3 Anti-Caspase-5 mAb M053-3 Anti-Caspase-7 mAb M032-3 Anti-Caspase-8 (Human) mAb M058-3 Anti-Caspase-8 (Human) mAb M054-3 Anti-Caspase-9 mAb M044-3 Anti-XIAP (MIHA/ILP-a) mAb
Product category	Research area Apoptosis

The availability is based on the information in Japan at 17:00, Apr 18, 2024 in JST.
The special price is shown in red color.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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