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HOME > Product search results > Code No. M059-3 Anti-Caspase-10 (Human) mAb

Code No. M059-3

Anti-Caspase-10 (Human) mAb

Availability (in Japan)

10 or more

(In Japan at 00:05,
Jul 28, 2021 in JST)


100 µg/100 µL

This antibody detects specifically 57 and 58 kDa of human caspase-10, 43 kDa and 30 kDa of human active caspase-10 on Western blotting (Park SJ et al. J Biol Chem. 279; 51057-51067 (2004)). This antibody reacts with isoforms caspase-10/a, 10/b and 10/d (Kischkel FC et al. J Biol Chem. 276, 46639-46646 (2001))
  • Western Blotting

Clonality Monoclonal Clone 4C1
Isotype (Immunized Animal) Mouse IgG1 κ
1-10 µg/mL  
Immunogen (Antigen) Recombinant full length Human FLICE2
Reactivity [Gene ID]

Human[843], Mouse(-)

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names CASP10, caspase 10, apoptosis-related cysteine peptidase, MCH4, ALPS2, FLICE2
Background Apoptosis is a major form of cell death characterized by several morphological features that include chromatin condensation and fragmentation, cell membrane blebbing, and formation of apoptotic bodies. These morphological changes occur via signaling pathway that leads to the recruitment and activation of caspases, a family of cysteine-containing, aspartate-specific proteases. Caspases exist as inactive proenzymes in cells and are activated through their processing into two subunits in response to apoptotic stimulation. Activated caspases cleave a variety of important cellular proteins, other caspases, and Bcl-2 family members, leading to a commitment to cell death. Caspase-10 (also known as Mch4, FLICE2 and ICE-LAP4) is a ~58 kDa protein. This caspase acts upstream of the apoptosis induced cascade. Once this caspase is activated by certain apoptotic stimuli, this protein may be responsible for the activation of the other caspases such as caspase-3, -4, -7, -8, and -9. This antibody was made against human-originated immunogen, and detects human caspase-10 specifically.
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Western Blotting

  1. Kischkel FC et al. Death receptor recruitment of endogenous caspase-10 and apoptosis initiation in the absence of caspase-8. J Biol Chem. 276, 46639-46 (2001)(PMID:11583996)
  2. Park SJ et al. J Biol Chem. Taxol induces caspase-10-dependent apoptosis. 279, 51057-67 (2004)(PMID:15452117)
  3. Koschny R et al. Bortezomib sensitizes primary human astrocytoma cells of WHO grades I to IV for tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis. Clin Cancer Res. 13, 3403-12 (2007)(PMID:17545549)
  4. Hyer ML et al. Apoptotic activity and mechanism of 2-cyano-3,12-dioxoolean-1,9-dien-28-oic-acid and related synthetic triterpenoids in prostate cancer. Cancer Res. 68, 2927-33 (2008)(PMID:18413762)
  5. Verbrugge I et al. Ionizing radiation modulates the TRAIL death-inducing signaling complex, allowing bypass of the mitochondrial apoptosis pathway. Oncogene 27, 574-84 (2008)(PMID:17684487)
  6. Strauss G et al. CD95 co-stimulation blocks activation of naive T cells by inhibiting T cell receptor signaling. J Exp Med. 206, 1379-93 (2009)(PMID:15452117)
  7. Lafont E et al. Caspase-10-dependent cell death in Fas/CD95 signalling is not abrogated by caspase inhibitor zVAD-fmk. PLoS One 5, e13638 (2010)(PMID:21049020)
  8. Cazanave SC et al. Death receptor 5 signaling promotes hepatocyte lipoapoptosis. J Biol Chem. 286, 39336-48 (2011)(PMID:21941003)
  9. Estornes Y et al. dsRNA induces apoptosis through an atypical death complex associating TLR3 to caspase-8. Cell Death Differ. 19, 1482-94 (2012)(PMID:22421964)
  10. Schleich K et al. Molecular architecture of the DED chains at the DISC: regulation of procaspase-8 activation by short DED proteins c-FLIP and procaspase-8 prodomain. Cell Death Differ. 23, 681-94 (2016)(PMID:26494467)
  11. Visiedo F et al. Characterization of NO-Induced Nitrosative Status in Human Placenta from Pregnant Women with Gestational Diabetes Mellitus. Oxid Med Cell Longev. 2017, 5629341 (2017)(PMID:28400911)
  12. Pešková L et al. Human Embryonic Stem Cells Acquire Responsiveness to TRAIL upon Exposure to Cisplatin. Stem Cells Int. 2019, 4279481 (2019) (PMID:30805008)
  13. Kumari R et al. Caspase-10 inhibits ATP-citrate lyase-mediated metabolic and epigenetic reprogramming to suppress tumorigenesis. Nat Commun. 10, 4255 (2019)(PMID:31534141)
  14. Lee SR et al. Accelerated degradation of cFLIPL and sensitization of the TRAIL DISC-mediated apoptotic cascade by pinoresinol, a lignan isolated from Rubia philippinensis. Sci Rep. 9,13505 (2019) (PMID:31534206)
  15. Elmallah MIY et al. Marine Actinomycetes-Derived Secondary Metabolites Overcome TRAIL-Resistance via the Intrinsic Pathway through Downregulation of Survivin and XIAP. Cells. 9, 1760 (2020)(PMID:32708048)
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Research area

  • The availability is based on the information in Japan at 00:05, Jul 28, 2021 in JST.
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  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
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