Anti-Caspase-8 (Human) mAb

Availability (in Japan)

10 or more

（In Japan at 17:00,
Apr 20, 2024 in JST）

Size

100 µL (1 mg/mL)

Citations

Data Sheet

SDS

Data	Western Blotting Western Blotting Immunoprecipitation
Clonality	Monoclonal	Clone	5D3
Isotype (Immunized Animal)	Mouse IgG2bκ
Applications	WB 1 µg/mL IP 1 µg/200 µL of cell extract from 2.5 x 10 ⁶ cells
Immunogen (Antigen)	Recombinant Human Caspase-8 (C-terminal 180-480 a.a.)
Reactivity [Gene ID]	Human[841], Mouse(-), Rat(-)
Storage buffer	PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	CASP8,CAP4, MACH, MCH5, FLICE, ALPS2B, Casp-8
Background	Caspase-8 (FLICE/MACH/Mch5) is a member of the ICE (interleukin-1 converting enzyme)/CED-3 family cysteine protease. It is the most upstream protease that receives the activation signal from the Fas (APO1/CD95) and TNFR1 (Tumor Necrosis Factor Receptor 1) to initiate the apoptotic protease cascade that leads to activation of ICE/CED-3 family proteases. Caspase-8 has high homologous region to the ICE/CED-3 family in C-terminal and two death effecter domains (DED) in N-terminal. Binding of caspase-8 to FADD (MORT1) through association of their DED, and consequent activation of the caspases by their proteolytic cleavage, are thought to be critical steps in the initiation of Fas- and TNFR1-induced apoptosis. Recently the inhibitor of Fas- and TNFR1-induced apoptosis is identified, called I-FLICE (FLIP/Casper/FLAME/CASH). I-FLICE has high homology to caspase-8 and it contains two DED, which interacts with caspase-8 and FADD, and potently inhibits Fas- and TNFR1-induced apoptosis.
Related products	M059-3 Anti-Caspase-10 (Human) mAb M087-3 Anti-Caspase-3 (Human) mAb M097-3 Anti-Caspase-3 (Human) mAb M029-3 Anti-Caspase-4 (Human) mAb M060-3 Anti-Caspase-5 mAb M053-3 Anti-Caspase-7 mAb M032-3 Anti-Caspase-8 (Human) mAb M054-3 Anti-Caspase-9 mAb
Citations	Tsujii H et al. Screening of cell death genes with a mammalian genome-wide RNAi library. J Biochem. 148, 157-70 (2010)(PMID:20421362) Western Blotting Tsuruma K et al. Glucocorticoid modulatory element-binding protein 1 binds to initiator procaspases and inhibits ischemia-induced apoptosis and neuronal injury. J Biol Chem. 281, 11397-404 (2006)(PMID:16497673) O'Reilly LA et al. Modifications and intracellular trafficking of FADD/MORT1 and caspase-8 after stimulation of T lymphocytes. Cell Death Differ. 11, 724-36 (2004)(PMID:15017386) Huang CR et al. Cisplatin augments FAS-mediated apoptosis through lipid rafts. Anticancer Res. 30, 2065-71 (2010)(PMID:20651352) Martin S et al. Cyclooxygenase-2 inhibition sensitizes human colon carcinoma cells to TRAIL-induced apoptosis through clustering of DR5 and concentrating death-inducing signaling complex components into ceramide-enriched caveolae. Cancer Res. 65, 11447-58 (2005)(PMID:16357153) Phillips DC et al. Sphingosine-induced apoptosis in rhabdomyosarcoma cell lines is dependent on pre-mitochondrial Bax activation and post-mitochondrial caspases. Cancer Res. 67, 756-64 (2007)(PMID:17234787)
Product category	Research area Apoptosis

The availability is based on the information in Japan at 17:00, Apr 20, 2024 in JST.
The special price is shown in red color.
Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.