Anti-Pseudouridine mAb

Availability (in Japan)

10 or more

（In Japan at 00:05,
Oct 9, 2024 in JST）

Size

100 µL (1 mg/mL)

Citations

Data Sheet

SDS

Data	Immunocytochemistry Immunohistochemistry
Clonality	Monoclonal	Clone	APU-6
Isotype (Immunized Animal)	Mouse IgG1 κ
Applications	IC 1 µg/mL IH 1 µg/mL ELISA* reported. （PMID: 2758683 / 8252470） Other(Immuno-Nothern blotting) Can be used. （PMID: 26606401）
Immunogen (Antigen)	KLH-conjugated pseudouridine
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	5-β-D-Ribofuranosyluracil, beta-pseudouridine, Ψ
Background	Pseudouridine (ψ), the first modified RNA base to be identified, presents mostly in non-coding RNAs such as tRNA, rRNA, snRNA and snoRNA. It is also reported that pseudouridine has been found in mRNA in both yeast and human by sequencing analysis. Replacing uridine to pseudouridine enhances structural stability of RNA, and is expected to affect rRNA processing, translation and pre-mRNA splicing.
Related products	D345-3 Anti-1-methyladenosine (m¹A) mAb D346-3 Anti-5-methylcytidine (m⁵C) mAb MI-11-3 Anti-Bromodeoxyuridine mAb PM077 Anti-5-hydroxymethylcytosine (5hmC) pAb MI-11-5 Anti-Bromodeoxyuridine mAb-PE M075-3 Mouse IgG1 (isotype control) M227-3 Anti-Digoxigenin (DIG) mAb M228-3 Anti-FITC mAb RN019M Anti-2,2,7-trimethylguanosine (m₃G/TMG) mAb RN016M Anti-7-methylguanosine (m⁷G)-Cap mAb RN017M Anti-7-methylguanosine (m⁷G) mAb PM098 Anti-Inosine pAb M218-3 Anti-5-hydroxymethylcytosine (5hmC) mAb
Citations	Other(Immuno-Nothern blotting) Mishima E et al. Immuno-Northern Blotting: Detection of RNA Modifications by Using Antibodies against Modified Nucleosides. PLoS One 10, e0143756 (2015)(PMID:26606401) Mishima E and Abe T. Immuno-Northern Blotting: Detection of Modified RNA Using Gel Separation and Antibodies to Modified Nucleosides. Methods Mol Biol. 1870, 179-187 (2019)(PMID:30539555) Stockert JA et al. Predictive value of pseudouridine in prostate cancer. Am J Clin Exp Urol. 7, 262-272 (2019)(PMID:31511832)
References	Masuda M et al. An immunohistochemical analysis for cancer of the esophagus using monoclonal antibodies specific for modified nucleosides. Cancer 72, 3571-8 (1993) [IHC-P, ELISA]（PMID: 8252470） Itoh K et al. Detection of elevated amounts of urinary pseudouridine in cancer patients by use of a monoclonal antibody. Clin Chim Acta 181, 305-15 (1989) [ELISA]（PMID: 2758683） Itoh K et al. Diagnostic use of anti-modified nucleoside monoclonal antibody. Tohoku J Exp Med. 168, 329-31 (1992)（PMID: 1306318）
Product category	Research area RNA-RNP Network Epigenetics

The availability is based on the information in Japan at 00:05, Oct 9, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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