Data |
Immunoprecipitation
Immunocytochemistry
Immunohistochemistry
|
Clonality |
Monoclonal
|
Clone |
AMA-2 |
Isotype
(Immunized Animal)
|
Mouse IgG2bκ
|
Applications |
- IP
- 15 µg/sample
- IC
- 1 µg/mL
- IH
- 0.1 µg/mL
- ELISA**
- Inhibition ELISA (Info. from the licenser. Not tested by MBL.)
- DB*
- reported. (PMID: 26863196 / 26863410)
- Other(Immuno-Nothern blotting)
- (PMID: 26606401)
|
Immunogen
(Antigen) |
KLH-conjugated 1-methyladenosine (KLH-m1A)
|
Storage buffer |
1 mg/mL in PBS/50% glycerol, pH 7.2
|
Storage temp. |
-20°C
|
Conjugate |
Unlabeled |
Manufacturer |
MBL |
Alternative names |
m1Ado
|
Background |
In eukaryote, newly synthesized RNA is chemically modified at specific nucleotide position after transcription. N1-methyladenosine (m1A) is one of major modified nucleosides present in most eukaryotic tRNAs and also found in yeast rRNAs. m1A affects structural stability of tRNA and its binding to polysome in translation. Urinary excretion of modified nucleosides including m1A is known to be increased in the patients with various cancers and AIDS. It is reported that the amount of urinary m1A correlates with m1A level in tissue from same patients with esophageal carcinoma. Moreover, elevated serum levels of m1A are also detected under stress conditions. |
Related products |
D346-3 Anti-5-methylcytidine (m5C) mAb PM077 Anti-5-hydroxymethylcytosine (5hmC) pAb MI-11-3 Anti-Bromodeoxyuridine mAb M077-3 Mouse IgG2b (isotype control) RN1005 RIP-Assay Kit for microRNA D347-3 Anti-Pseudouridine mAb RN127PW Anti-NSUN2 (Human) pAb RN128PW Anti-TRMT6 (Human) pAb M227-3 Anti-Digoxigenin (DIG) mAb M218-3 Anti-5-hydroxymethylcytosine (5hmC) mAb M228-3 Anti-FITC mAb RN019M Anti-2,2,7-trimethylguanosine (m3G/TMG) mAb RN016M Anti-7-methylguanosine (m7G)-Cap mAb RN017M Anti-7-methylguanosine (m7G) mAb PM098 Anti-Inosine pAb
|
Citations |
Dot Blotting - Dominissini D et al. The dynamic N(1)-methyladenosine methylome in eukaryotic messenger RNA. Nature 530, 441-6 (2016)(PMID:26863196)
- Li X et al. Transcriptome-wide mapping reveals reversible and dynamic N(1)-methyladenosine methylome. Nat Chem Biol. 12, 311-6 (2016)(PMID:26863410)
- Liu F et al. ALKBH1-Mediated tRNA Demethylation Regulates Translation. Cell 167, 816-828.e16. (2016)(PMID:27745969)
- Ueda Y et al. AlkB homolog 3-mediated tRNA demethylation promotes protein synthesis in cancer cells. Sci Rep. 7, 42271 (2017)(PMID:28205560)
Northern Blotting - Elkordy A et al. tiRNAs as a novel biomarker for cell damage assessment in in vitro ischemia-reperfusion model in rat neuronal PC12 cells. Brain Res. 1714, 8-17 (2019)(PMID:30779912)
Immunoprecipitation - Dominissini D et al. The dynamic N(1)-methyladenosine methylome in eukaryotic messenger RNA. Nature 530, 441-6 (2016)(PMID:26863196)
- Li X et al. Transcriptome-wide mapping reveals reversible and dynamic N(1)-methyladenosine methylome. Nat Chem Biol. 12, 311-6 (2016)(PMID:26863410)
- Waku et al. NML-mediated rRNA base methylation links ribosomal subunit formation to cell proliferation in a p53-dependent manner. J. Cell Sci. 129, 2382-93 (2016)(PMID:27149924)
- Safra M et al. The m1A landscape on cytosolic and mitochondrial mRNA at single-base resolution. Nature 551, 251-255 (2017)(PMID:29072297)
- Li X et al. Base-Resolution Mapping Reveals Distinct m1A Methylome in Nuclear- and Mitochondrial-Encoded Transcripts. Mol Cell 68, 993-1005 (2017)(PMID:29107537)
Immunocytochemistry - Ueda Y et al. AlkB homolog 3-mediated tRNA demethylation promotes protein synthesis in cancer cells. Sci Rep. 7, 42271 (2017)(PMID:28205560)
Other(Immuno-Nothern blotting) - Mishima E et al. Immuno-Northern Blotting: Detection of RNA Modifications by Using Antibodies against Modified Nucleosides. PLoS One 10, e0143756 (2015)(PMID:26606401)
- Alaa E et al. Stress-induced tRNA cleavage and tiRNA generation in rat neuronal PC12 cells. J Neurochem. (2018) In press.(PMID:29431851)
- Mishima E and Abe T. Immuno-Northern Blotting: Detection of Modified RNA Using Gel Separation and Antibodies to Modified Nucleosides. Methods Mol Biol. 1870, 179-187 (2019)
(PMID:30539555)
- Sato K et al. Stress Induced tRNA Halves (tiRNAs) as Biomarkers for Stroke and Stroke Therapy; Pre-clinical Study. Neuroscience. 434, 44-54 (2020)(PMID:32200075)
|
References |
- Itoh K et al. Preparation of a monoclonal antibody specific for 1-methyladenosine and its application for the detection of elevated levels of 1-methyladenosine in urines from cancer patients. Jpn J Cancer Res. 79, 1130-8 (1988)(PMID: 3143701)
- Itoh K et al. Diagnostic use of anti-modified nucleoside monoclonal antibody. Tohoku J Exp Med. 168, 329-31 (1992)1306318(PMID: 1306318)
- Masuda M et al. An immunohistochemical analysis for cancer of the esophagus using monoclonal antibodies specific for modified nucleosides.Cancer 72, 3571-8 (1993)(PMID: 8252470)
- Mishima E et al. Conformational Change in Transfer RNA Is an Early Indicator of Acute Cellular Damage. J Am Soc Nephrol. 25, 2316-26 (2014)(PMID: 24833129)
- Chi KR. The RNA code comes into focus. Nature 542, 503-506 (2017)(PMID: 28230128)
|
Product category |
- Research area
- RNA-RNP Network
Epigenetics Cancer Cellular stress
|