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HOME > Product search results > Code No. CM004-3 Anti-SynCAM (TSLC1/CADM1) mAb

Code No.　CM004-3

Anti-SynCAM (TSLC1/CADM1) mAb

Availability (in Japan)

10 or more

（In Japan at 00:05,
Apr 19, 2024 in JST）

Size

100 µL (1 mg/mL)

Data

  • Western Blotting

  • Immunoprecipitation

  • Flow Cytometry
Clonality Monoclonal Clone 3E1
Isotype (Immunized Animal) Chicken IgY
Applications
WB
1 µg/mL  
IP
10 µg/200 µL of cell extract from 5x106 cells  
FCM
1 µg/mL (final concentration)  
IC*
reported.  （PMID: 16394014 / 17314315 / 19643986）
IH
10 µg/mL (Heat treatment is necessary for paraffin embedded sections.)  
Immunogen (Antigen) Recombinant SynCAM-Fc fusion protein
Reactivity [Gene ID]

Human[23705], Mouse*[54725](PMID: 19435791/23741316/24753442), Rat*[363058](PMID: 27756895), Horse*[100062374](PMID: 27524460), Porcine*[106504882](PMID: 28890720)
Storage buffer PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names cell adhesion molecule 1, BL2, IGSF4, IGSF4A, NECL2, Necl-2, RA175, ST17, sTSLC-1, sgIGSF
Background SynCAM (Synaptic Cell Adhesion Molecule), also known as TSLC1 (tumor suppressor in lung cancer-1), SgIGSF, or CADM1, is a homophilic, transmembrane Ig-domain containing protein with intracellular PDZ protein-binding motifs. Although originally identified as a tumor suppressor of small lung cell carcinomas, SynCAM appears to be primarily involved in intracellular adhesion and synapse formation. The majority of SynCAM is localized to synaptic sites where it initiates synaptic assembly and synapse differentiation throughout the central nervous system. SynCAM also mediates the cellular adhesion of spermatogenic cells to Sertoli cells and mast cells to fibroblasts.
Related products CM005-3 Anti-SynCAM (TSLC1/CADM1) (Human) mAb
PM084 Normal Chicken IgY
PM084-4 Normal Chicken IgY-FITC
PM084-5 Normal Chicken IgY-PE
CM004-4 Anti-SynCAM (TSLC1/CADM1) mAb-FITC
CM004-5 Anti-SynCAM (TSLC1/CADM1) mAb-PE
CM004-6 Anti-SynCAM (TSLC1/CADM1) mAb-Biotin
CM004-A64 Anti-SynCAM (TSLC1/CADM1) mAb-Alexa Fluor™ 647
Citations

Western Blotting

  1. Fogel AI et al. SynCAMs organize synapses through heterophilic adhesion. J Neurosci. 27, 12516-30 (2007)(PMID:18003830)
  2. Hagiyama M et al. Expression of a soluble isoform of cell adhesion molecule 1 in the brain and its involvement in directional neurite outgrowth. Am J Pathol. 174, 2278-89 (2009)(PMID:19435791)
  3. Gao Y, Lui WY. Transforming growth factor-β1 (TGF-β1) regulates cell junction restructuring via Smad-mediated repression and clathrin-mediated endocytosis of nectin-like molecule 2 (Necl-2). PLoS One 8, e64316 (2013)(PMID:23741316)
  4. Ribic A et al. Structural organization and function of mouse photoreceptor ribbon synapses involve the immunoglobulin protein synaptic cell adhesion molecule 1. J Comp Neurol. 522, 900-20 (2014)(PMID:23982969)
  5. Nikitczuk JS et al. N-cadherin regulates molecular organization of excitatory and inhibitory synaptic circuits in adult hippocampus in vivo. Hippocampus 24, 943-962 (2014)(PMID:24753442)
  6. Cheadle L, Biederer T. Activity-dependent regulation of dendritic complexity by semaphorin 3A through Farp1. J Neurosci. 34, 7999-8009 (2014)(PMID:24899721)
  7. Rathjen T et al. Regulation of body weight and energy homeostasis by neuronal cell adhesion molecule 1. Nat Neurosci. 20, 1096-1103 (2017)(PMID:28628102)

Immunoprecipitation

  1. Fogel AI et al. SynCAMs organize synapses through heterophilic adhesion. J Neurosci. 27, 12516-30 (2007)(PMID:18003830)
  2. Hollins F et al. Human airway smooth muscle promotes human lung mast cell survival, proliferation, and constitutive activation: cooperative roles for CADM1, stem cell factor, and IL-6. J Immunol. 181, 2772-80 (2008)(PMID:18684968)
  3. Hagiyama M et al. Expression of a soluble isoform of cell adhesion molecule 1 in the brain and its involvement in directional neurite outgrowth. Am J Pathol. 174, 2278-89 (2009)(PMID:19435791)

Flow Cytometry

  1. Yang W et al. Human lung mast cells adhere to human airway smooth muscle, in part, via tumor suppressor in lung cancer-1. J Immunol. 176, 1238-43 (2006)(PMID:16394014)
  2. Zhang C et al. Extracellular CADM1 interactions influence insulin secretion by rat and human islet β-cells and promote clustering of syntaxin-1. Am J Physiol Endocrinol Metab. 310, E874-85 (2016)(PMID:27072493)
  3. Ziegler A et al. Identification and characterization of equine blood plasmacytoid dendritic cells. Dev Comp Immunol. 65, 352-357 (2016)(PMID:27524460)
  4. Braun RO et al. Porcine B Cell Subset Responses to Toll-like Receptor Ligands. Front Immunol. 8, 1044 (2017)(PMID:28890720)
  5. Balan S et al. Large-Scale Human Dendritic Cell Differentiation Revealing Notch-Dependent Lineage Bifurcation and Heterogeneity. Cell Rep. 24, 1902-1915.e6 (2018)(PMID:30110645)
  6. Oka T et al. Metabolic abnormalities in adult T-cell leukemia/lymphoma and induction of specific leukemic cell death using photodynamic therapy. Sci Rep. 8, 14979 (2018)(PMID:30297858)
  7. Parra-Sánchez H et al. Analysis of Swine Conventional Dendritic Cells, DEC205+CD172a+/-CADM1+, from Blood and Spleen in Response to PRRSV and PEDV. Viruses. 11, pii: E1001 (2019)(PMID:31683569)
  8. Bustamante-Córdova L et al. Production of a full chimeric mouse x pig anti-porcine DEC205 receptor recombinant antibody. J Immunol Methods. 489, 112911 (2021)(PMID:33186587)

Immunocytochemistry

  1. Yang W et al. Human lung mast cells adhere to human airway smooth muscle, in part, via tumor suppressor in lung cancer-1. J Immunol. 176, 1238-43 (2006)(PMID:16394014)
  2. Wakayama T et al. Heterophilic binding of the adhesion molecules poliovirus receptor and immunoglobulin superfamily 4A in the interaction between mouse spermatogenic and Sertoli cells. Biol Reprod. 76, 1081-90 (2007)(PMID:17314315)
  3. Hollins F et al. Human airway smooth muscle promotes human lung mast cell survival, proliferation, and constitutive activation: cooperative roles for CADM1, stem cell factor, and IL-6. J Immunol. 181, 2772-80 (2008)(PMID:18684968)
  4. Rademacher N et al. MPP2 is a postsynaptic MAGUK scaffold protein that links SynCAM1 cell adhesion molecules to core components of the postsynaptic density. Sci Rep. 6, 35283 (2016)(PMID:27756895)

Immunohistochemistry

  1. Fujita E et al. Loss of partitioning-defective-3/isotype-specific interacting protein (par-3/ASIP) in the elongating spermatid of RA175 (IGSF4A/SynCAM)-deficient mice. Am J Pathol. 171, 1800-10 (2007)(PMID:18055550)
  2. Hasstedt SJ et al. Cell adhesion molecule 1: a novel risk factor for venous thrombosis. Blood. 114, 3084-91 (2009)(PMID:19643986)
  3. Ribic A et al. Structural organization and function of mouse photoreceptor ribbon synapses involve the immunoglobulin protein synaptic cell adhesion molecule 1. J Comp Neurol. 522, 900-20 (2014)(PMID:23982969)
  4. Park KA et al. Excitatory Synaptic Drive and Feedforward Inhibition in the Hippocampal CA3 Circuit Are Regulated by SynCAM 1. J Neurosci. 36, 7464-75 (2016)(PMID:27413156)
  5. Rathjen T et al. Regulation of body weight and energy homeostasis by neuronal cell adhesion molecule 1. Nat Neurosci. 20, 1096-1103 (2017)(PMID:28628102)

ELISA

  1. Nakahata S et al. Clinical significance of soluble CADM1 as a novel marker for adult T-cell leukemia/lymphoma. Haematologica.in press. (PMID:32054656)
Product category
Research area
Cancer
Neuroscience
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  • The availability is based on the information in Japan at 00:05, Apr 19, 2024 in JST.
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  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
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