Anti-7-methylguanosine (m⁷G) mAb

Availability (in Japan)

10 or more

（In Japan at 17:00,
Apr 19, 2024 in JST）

Size

200 µL (1 mg/mL)

Citations

Data Sheet

SDS

This antibody (clone 4141-13) reacts with both 5′-terminal and internal 7-methylguanosine (m⁷G) in RNA.

Data	Immunoprecipitation Immunoprecipitation Dot Blotting
Clonality	Monoclonal	Clone	4141-13
Isotype (Immunized Animal)	Mouse IgG2aκ
Applications	IP 10 µg/sample IC Can be used. ELISA Can be used. DB 1 µg/mL Other(Northwestern blotting) reported. （PMID: 29983320）
Immunogen (Antigen)	Carrier protein-conjugated 7-methylguanosine (m⁷G)-Cap analogue
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Background	The 7-methylguanosine (m⁷G) is a modified nucleoside commonly found in eukaryotes. The m⁷G modification present on the 5′-end of mRNA and on some ncRNA synthesized by RNA polymerase II is called m⁷G cap. It plays a role in transcription, splicing, polyadenylation, nuclear export, stabilization, and translation. In addition, other m⁷G modifications are also found in the tRNA or rRNA. The m⁷G₄₆ modification occurs in bacterial and eukaryotic tRNAs. The m⁷G₁₅₇₅ modification in the 18S rRNA is evolutionarily conserved in eukaryotes.
Related products	RN016M Anti-7-methylguanosine (m⁷G)-Cap mAb RN1005 RIP-Assay Kit for microRNA D347-3 Anti-Pseudouridine mAb D345-3 Anti-1-methyladenosine (m¹A) mAb D346-3 Anti-5-methylcytidine (m⁵C) mAb PM077 Anti-5-hydroxymethylcytosine (5hmC) pAb MI-11-3 Anti-Bromodeoxyuridine mAb MI-11-5 Anti-Bromodeoxyuridine mAb-PE M218-3 Anti-5-hydroxymethylcytosine (5hmC) mAb M227-3 Anti-Digoxigenin (DIG) mAb M228-3 Anti-FITC mAb RN019M Anti-2,2,7-trimethylguanosine (m₃G/TMG) mAb RN006M Anti-EIF4E mAb RN001P Anti-EIF4E pAb RN126PW Anti-AHCY (SAHH) pAb RN124PW Anti-RNMT (Human) pAb PM098 Anti-Inosine pAb
Citations	Immunoprecipitation Lin S et al. Mettl1/Wdr4-Mediated m⁷G tRNA Methylome Is Required for Normal mRNA Translation and Embryonic Stem Cell Self-Renewal and Differentiation. Mol Cell 71, 244-255.e5 (2018)(PMID:29983320) RNP Immunoprecipitation Pandolfini L et al. METTL1 Promotes let-7 MicroRNA Processing via m7G Methylation. Mol Cell. pii: S1097-2765(19)30269-2 (2019)(PMID:31031083) Other(Northwestern blotting) Lin S et al. Mettl1/Wdr4-Mediated m⁷G tRNA Methylome Is Required for Normal mRNA Translation and Embryonic Stem Cell Self-Renewal and Differentiation. Mol Cell 71, 244-255.e5 (2018)(PMID:29983320)
References	Maden BE, Salim M. The methylated nucleotide sequences in HELA cell ribosomal RNA and its precursors. J Mol Biol. 88, 133-52 (1974)（PMID: 4374549 ） Cowling VH. Regulation of mRNA cap methylation. Biochem J. 425, 295-302 (2009)（PMID: 20025612） Cubrilo S et al. The aminoglycoside resistance methyltransferase Sgm impedes RsmF methylation at an adjacent rRNA nucleotide in the ribosomal A site. RNA 15, 1492-7 (2009)（PMID: 19509304） Chen P et al. Transfer RNA modifications and genes for modifying enzymes in Arabidopsis thaliana. BMC Plant Biol. 10, 201 (2010)（PMID: 20836892） Filonava L et al. A novel cause for primordial dwarfism revealed: defective tRNA modification. Genome Biol. 16, 216 (2015)（PMID: 26429597 ） Zorbas C et al. The human 18S rRNA base methyltransferases DIMT1L and WBSCR22-TRMT112 but not rRNA modification are required for ribosome biogenesis. Mol Biol Cell. 26, 2080-95 (2015)（PMID: 25851604 ）
Product category	Research area RNA-RNP Network Epigenetics Brands RiboCluster Profiler™

The availability is based on the information in Japan at 17:00, Apr 19, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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