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HOME > Product search results > Code No. RN011P Anti-PTBP1 (Human) pAb

Code No.　RN011P

Anti-PTBP1 (Human) pAb

Availability (in Japan)

10 or more

（In Japan at 00:05,
Apr 20, 2024 in JST）

Size

200 µL (1 mg/mL)

Data

  • Western Blotting

  • Immunoprecipitation

  • RNP Immunoprecipitation
Clonality Polyclonal Clone Polyclonal
Isotype (Immunized Animal) Rabbit Ig (aff.)
Applications
WB
1:1,000  
IP
5 µg/250 µL of cell extract from 2.5 x 106 cells  
IC*
reported.  （PMID: 24865424 / 26990993）
RIP
15 µg/500 µL of cell extract from 6 x 106 cells  
Other(eCLIP)
reported.  （PMID: 27018577）
Immunogen (Antigen) KLH-conjugated synthetic peptide KFKGDSRSAGVPSR (46-59 a. a.)
Reactivity [Gene ID]

Human[5725], Mouse(-), Rat(-), Hamster(-)
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names polypyrimidine tract binding protein 1, HNRNP-I, HNRNPI, HNRPI
Background The polypyrimidine tract binding protein 1 (PTBP1), a 57 kDa RNA binding protein also known as hnRNP1, has been implicated in multiple aspect of mRNA metabolism, including regulation of alternative splicing, internal ribosome entry site-driven translation, mRNA localization, and polyadenylation. Four of tandem RNA recognition motif domains give PTBP1 a strong binding activity to RNA. Posttranscriptional regulation of gene expression by PTBP1 has been reported on many genes, including CD154, insulin, VEGF and Human inducible nitric oxide synthase.
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Citations

Western Blotting

  1. Ferrarese R et al. Lineage-specific splicing of a brain-enriched alternative exon promotes glioblastoma progression. J Clin Invest. 124, 2861-74 (2014)(PMID:24865424)
  2. Liu W et al. Hepatitis B virus core protein inhibits Fas-mediated apoptosis of hepatoma cells via regulation of mFas/FasL and sFas expression. FASEB J. 29, 1113-23 (2015)(PMID:25466893)
  3. Cui J, Placzek WJ. PTBP1 modulation of MCL1 expression regulates cellular apoptosis induced by antitubulin chemotherapeutics. Cell Death Differ. 23, 1681-90 (2016)(PMID:27367564)
  4. Nussbacher JK, Yeo GW. Systematic Discovery of RNA Binding Proteins that Regulate MicroRNA Levels. Mol Cell. 69, 1005-1016.e7 (2018)(PMID:29547715)
  5. Cui J, Placzek WJ. PTBP1 enhances miR-101-guided AGO2 targeting to MCL1 and promotes miR-101-induced apoptosis. Cell Death Dis. 9, 552 (2018)(PMID:29748555)

Immunoprecipitation

  1. Ferrarese R et al. Lineage-specific splicing of a brain-enriched alternative exon promotes glioblastoma progression. J Clin Invest. 124, 2861-74 (2014)(PMID:24865424)

Immunocytochemistry

  1. Ferrarese R et al. Lineage-specific splicing of a brain-enriched alternative exon promotes glioblastoma progression. J Clin Invest. 124, 2861-74 (2014)(PMID:24865424)
  2. Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993)

RNP Immunoprecipitation

  1. Ferrarese R et al. Lineage-specific splicing of a brain-enriched alternative exon promotes glioblastoma progression. J Clin Invest. 124, 2861-74 (2014)(PMID:24865424)
  2. Cui J, Placzek WJ. PTBP1 modulation of MCL1 expression regulates cellular apoptosis induced by antitubulin chemotherapeutics. Cell Death Differ. 23, 1681-90 (2016)(PMID:27367564)
  3. Wang ZN et al. High expression of PTBP1 promote invasion of colorectal cancer by alternative splicing of cortactin. Oncotarget 8, 36185-36202 (2017)(PMID:28404950)

Other(eCLIP)

  1. Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993)
  2. Van Nostrand EL et al. Robust transcriptome-wide discovery of RNA-binding protein binding sites with enhanced CLIP (eCLIP). Nat Methods. 13, 508-14 (2016)(PMID:27018577)
Product category
Research area
RNA-RNP Network
Brands
RiboCluster Profiler™
この製品に関するお問い合わせをする

  • The availability is based on the information in Japan at 00:05, Apr 20, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.