Anti-EIF4E pAb

Availability (in Japan)

10 or more

（In Japan at 17:00,
Apr 16, 2024 in JST）

Size

200 µL

Citations

Data Sheet

SDS

Data	Western Blotting Immunoprecipitation RNP Immunoprecipitation
Clonality	Polyclonal	Clone	Polyclonal
Isotype (Immunized Animal)	Rabbit Ig (aff.)
Applications	WB 1:1,000 IP 5 µL/250 µL of cell extract from 2.5 x 10⁶ cells IC Can be used. RIP 15 µL/500 µL of cell extract from 4.5 x 10⁶ cells
Immunogen (Antigen)	KLH-conjugated synthetic peptide MATVEPETTPTPNPPTTEEEKTESNQEVANPEHYIKH (1-37 a.a.)
Reactivity [Gene ID]	Human[1977](PMID: 22397984), Mouse[13684], Rat[117045], Hamster[100773768]
Storage buffer	200 µL volume of PBS containing 50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	eukaryotic translation initiation factor 4E, CBP, EIF4E1, EIF4EL1/If4e, eIF-4E, EG668879, Eif4e-ps
Background	The eukaryotic initiation factor 4E (eIF4E) is a key regulatory component that anchors the mRNA cap-binding complex (eIF4F) to the 5’ end of capped mRNAs. eIF3, the poly (A)-binding protein, and the eIF4 proteins recruit mRNA to the 43S initiation complex to form the 48S initiation complex. The eIF4 proteins consist of eIF4A; RNA helicase (46 kDa), eIF4B; RNA binding and RNA annealing protein (70 kDa), eIF4E (25 kDa); cap binding protein, eIF4H; work with eIF4B to stimulate eIF4A helicase activity (25 kDa), and eIF4G; co-localize all other proteins necessary for mRNA recruitment on the 40S subunit. As a principal initiation factor, eIF4E has the potential to influence expression of every protein in the cell. mRNA subset associated with eIF4E in p19 cell differed from mRNA subset associated with HuB or poly A binding protein, suggested that they reflect distinct functional subset of mRNAs whose expression is regulated differently at the level of translation.
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Citations	Western Blotting Fukao A et al. MicroRNAs Trigger Dissociation of eIF4AI and eIF4AII from Target mRNAs in Humans. Mol Cell 56, 79-89 (2014)(PMID:25280105) RNP Immunoprecipitation Thompson K et al. Acute adaptive responses of central sensorimotor neurons after spinal cord injury. Translational Neuroscience 1, 268-78 (2010) Hayman TJ et al. Translation initiation factor eIF4E is a target for tumor cell radiosensitization. Cancer Res. 72, 2362-72 (2012)(PMID:22397984) Culjkovic-Kraljacic B et al. Combinatorial targeting of nuclear export and translation of RNA inhibits aggressive B-cell lymphomas. Blood 127, 858-68 (2016)(PMID:26603836) Rissland OS et al. The influence of microRNAs and poly(A) tail length on endogenous mRNA-protein complexes. Genome Biol. 18, 211 (2017)(PMID:29089021) Zahreddine HA et al. The eukaryotic translation initiation factor eIF4E harnesses hyaluronan production to drive its malignant activity. Elife. 6, e29830 (2017)(PMID:29111978)
Product category	Research area RNA-RNP Network Cancer Brands RiboCluster Profiler™

The availability is based on the information in Japan at 17:00, Apr 16, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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