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HOME > Product search results > Code No. PM054 Anti-α-Tubulin pAb

Code No. PM054

Anti-α-Tubulin pAb

Availability (in Japan)

1-9

(In Japan at 00:05,
Jul 28, 2021 in JST)

Size

100 µL

Data
  • Western Blotting

  • Immunoprecipitation

  • Immunocytochemistry

Clonality Polyclonal Clone Polyclonal
Isotype (Immunized Animal) Rabbit Ig (aff.)
Applications
WB
1:1,000  
IP
2 µL/200 µL of cell extract from 2 x 106 cells  
IC
1:200  
Immunogen (Antigen) N-terminal α-Tubulin synthetic peptide
Reactivity [Gene ID]

Human[7846], Mouse[22142], Rat[64158], Hamster[100682527], Chicken[416694], Fruit fly*(PMID: 26718004/27559081/28972163)

Storage buffer PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Background Microtubules are one of the components of the cytoskeleton, which performs essential and diverse functions within eukaryotic cells. Microtubules are composed of a heterodimer of alpha and beta tubulins. Tubulin is a GTP-binding protein, and extension and shortening of the microtubules are regulated by binding/hydrolysis of GTP.
Related products PM054-7 Anti-α-Tubulin pAb-HRP-DirecT
PM088 Anti-Vinculin pAb
M175-3 Anti-α-Tubulin mAb
M177-3 Anti-β-Actin mAb
Citations

Western Blotting

  1. Geng Y et al. Contribution of the C-terminal Regions of Promyelocytic Leukemia Protein (PML) Isoforms II and V to PML Nuclear Body Formation. J Biol Chem. 287, 30729-42 (2012)(PMID:22773875)
  2. Cao JX et al. MiR-630 inhibits proliferation by targeting CDC7 kinase, but maintains the apoptotic balance by targeting multiple modulators in human lung cancer A549 cells. Cell Death Dis. 5,e1426  (2014)(PMID:25255219)
  3. Horii T et al. p53 suppresses tetraploid development in mice. Sci Rep. 5, 8907 (2015)(PMID:25752699)
  4. Xu D et al. Modification of BECN1 by ISG15 plays a crucial role in autophagy regulation by type I IFN/interferon. Autophagy 11, 617-28 (2015)(PMID:25906440)
  5. Xu D et al. Phosphorylation and activation of ubiquitin-specific protease-14 by Akt regulates the ubiquitin-proteasome system. Elife 4, e10510 (2015)(PMID:26523394)
  6. Wang H et al. Antagonistic roles of Nibbler and Hen1 in modulating piRNA 3' ends in Drosophila. Development 143, 530-9 (2016)(PMID:26718004)
  7. Li H et al. Ultra-deep sequencing of ribosome-associated poly-adenylated RNA in early Drosophila embryos reveals hundreds of conserved translated sORFs. DNA Res. 23, 571-580 (2016)(PMID:27559081)
  8. Xu D et al. USP25 regulates Wnt signaling by controlling the stability of tankyrases. Genes Dev. 31, 1024-1035 (2017)(PMID:28619731)
  9. Shibutani M et al. Arid1b Haploinsufficiency Causes Abnormal Brain Gene Expression and Autism-Related Behaviors in Mice. Int J Mol Sci. 18, E1872 (2017)(PMID:28867767)
  10. Murakami A et al. An N-terminal di-proline motif is essential for fatty acid-dependent degradation of Δ9-desaturase in Drosophila. J Biol Chem. 292, 19976-19986 (2017)(PMID:28972163)
  11. Fu W et al. A novel acridine derivative, LS-1-10 inhibits autophagic degradation and triggers apoptosis in colon cancer cells. Cell Death Dis. 8, e3086 (2017)(PMID:28981103)
  12. Zhu K et al. Necroptosis promotes cell-autonomous activation of proinflammatory cytokine gene expression. Cell Death Dis. 9, 500 (2018)(PMID:29703889)
  13. Makise M et al. The role of vimentin in the tumor marker Nup88-dependent multinucleated phenotype. BMC Cancer 18, 519 (2018)(PMID:29724197)
  14. Sakane H, Akasaki K. The Major Lysosomal Membrane Proteins LAMP-1 and LAMP-2 Participate in Differentiation of C2C12 Myoblasts. Biol Pharm Bull. 41, 1186-1193 (2018)(PMID:30068868)
  15. Ikawa K, Sugimura K. AIP1 and cofilin ensure a resistance to tissue tension and promote directional cell rearrangement. Nat Commun. 9, 3295 (2018)(PMID:30202062)
  16. Sun X et al. Distinct multilevel misregulations of Parkin and PINK1 revealed in cell and animal models of TDP-43 proteinopathy. Cell Death Dis. 9, 953 (2018)(PMID:30237395)
  17. Cao JX, Lu Y, Targeting CDC7 improves sensitivity to chemotherapy of esophageal squamous cell carcinoma. Onco Targets Ther. 12, 63-74 (2019)(PMID:30588031)
  18. Aoki T et al. Intraflagellar transport 20 promotes collective cancer cell invasion by regulating polarized organization of Golgi-associated microtubules. Cancer Sci. 110, 1306-1316 (2019)(PMID:30742741)
  19. Yamada S, Sakakibara SI. Expression profile of the STAND protein Nwd1 in the developing and mature mouse central nervous system. J Comp Neurol. 526, 2099-2114 (2018)(PMID:30004576)
  20. Kanazawa T et al. Effect of Ovariectomy and Ovarian Hormone Administration on Hepatic Autophagy in Female Rats. J Nutr Sci Vitaminol (Tokyo). 65, 357-361 (2019)(PMID:31474686)
  21. Kato H et al. Development of a recombinant replication-deficient rabies virus-based bivalent-vaccine against MERS-CoV and rabies virus and its humoral immunogenicity in mice. PLoS One. 14, e0223684 (2019)(PMID:31589656)
  22. Tsuru E et al. Complexin 2 regulates secretion of immunoglobulin in antibody-secreting cells. Immun Inflamm Dis. 7, 318-325 (2019) (PMID:31691534)
  23. Kamohara A et al. IgG immune complexes with Staphylococcus aureus protein A enhance osteoclast differentiation and bone resorption by stimulating Fc receptors and TLR2. Int Immunol. 32, 89-104 (2019)(PMID:31713625)
  24. Kurashima K et al. SMARCA4 deficiency-associated heterochromatin induces intrinsic DNA replication stress and susceptibility to ATR inhibition in lung adenocarcinoma. NAR Cancer. 2, zcaa005 (2020)
  25. Yamamoto YH et al. ERdj8 governs the size of autophagosomes during the formation process. J Cell Biol. 219, e201903127 (2020)(PMID:32492081)
  26. Miki H et al. Genetic and epigenetic regulation of osteopontin by cyclic adenosine 3' 5'-monophosphate in osteoblasts. Gene. 763, 145059 (2020)(PMID:32858177)

Immunocytochemistry

  1. Taniuchi K et al. KIF20A-mediated RNA granule transport system promotes the invasiveness of pancreatic cancer cells. Neoplasia 16, 1082-93 (2014)(PMID:25499221)
  2. Chinen T et al. The γ-tubulin-specific inhibitor gatastatin reveals temporal requirements of microtubule nucleation during the cell cycle. Nat Commun. 6, 8722 (2015)(PMID:26503935)
  3. Ma K et al. PTK2-mediated degradation of ATG3 impedes cancer cells susceptible to DNA damage treatment. Autophagy 13, 579-591 (2017)(PMID:28103122)
  4. Aoki T et al. Intraflagellar transport 20 promotes collective cancer cell invasion by regulating polarized organization of Golgi-associated microtubules. Cancer Sci. 110, 1306-1316 (2019)(PMID:30742741)
Product category
Tools
Control antibodies
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  • The availability is based on the information in Japan at 00:05, Jul 28, 2021 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.