Anti-β-galactosidase pAb

Availability (in Japan)

10 or more

（In Japan at 00:05,
Apr 20, 2024 in JST）

Size

100 µL

Citations

Data Sheet

SDS

Data	Western Blotting Immunoprecipitation Immunocytochemistry Immunohistochemistry
Clonality	Polyclonal	Clone	polyclonal
Isotype (Immunized Animal)	Rabbit IgG
Applications	WB 1:1,000 IP 1 µL/sample IC 1:100 IH 1:200
Immunogen (Antigen)	E. coli full-length β-galactosidase
Storage buffer	PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Background	β-galactosidase is a homo-tetrameric enzyme, with each subunit having a molecular weight of 116 kDa. Eukaryotic genes are often expressed as fusion protein by the β-galactosidase (lacZ) gene, resulting in the expression of a fusion hybrid with b-galactosidase. Anti-β-galactosidase antibody provides a simple method to isolate fusion proteins directly from crude bacterial lysates, using immunoaffinity chromatography or immunoprecipitation. Anti-β-galactosidase can also be used for the immunocytochemical detection of β-galactosidase in cells and tissues that express transfected bacterial lacZ gene or β-galactosidase fusion protein.
Related products	M094-3 Anti-β-galactosidase mAb M203-3 Anti-β-galactosidase mAb
Citations	Vaish V et al. Lentivirus-mediated somatic recombination and development of a novel mouse model for sporadic colorectal cancer. Genes Chromosomes Cancer. 55, 577-90 (2016)(PMID:27037682) Immunohistochemistry Otsu K et al. The Semaphorin 4D-RhoA-Akt Signal Cascade Regulates Enamel Matrix Secretion in Coordination With Cell Polarization During Ameloblast Differentiation. J Bone Miner Res. 31, 1943-1954 (2016)(PMID:27218883)
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The availability is based on the information in Japan at 00:05, Apr 20, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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