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HOME > Product search results > Code No. M214-3 Anti-mini-AID-tag mAb

Code No.　M214-3

Anti-mini-AID-tag mAb

Availability (in Japan)

10 or more

（In Japan at 00:05,
Apr 17, 2024 in JST）

Size

100 µL (1 mg/mL)

Data

  • Western Blotting

  • Western Blotting

  • Immunoprecipitation
Clonality Monoclonal Clone 1E4
Isotype (Immunized Animal) Mouse IgG2aκ
Applications
WB
1-5 µg/mL  
IP
5 µg/sample  
IC
5 µg/mL  
Immunogen (Antigen) 17 aa sequence of Auxin Inducible Degron Internal region (mini-AID-tag)
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names mini-Degron-tag, mini-Auxin inducible Degron-tag
Related products PD048 Anti-OsTIR1 pAb
Citations

Western Blotting

  1. Natsume T et al. Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors. Cell Rep. 15, 210-8 (2016)(PMID:27052166)
  2. Schuller AP et al. eIF5A Functions Globally in Translation Elongation and Termination. Mol Cell. 66, 194-205.e5 (2017)(PMID:28392174)
  3. Natsume T et al. Acute inactivation of the replicative helicase in human cells triggers MCM8-9-dependent DNA synthesis. Genes Dev. 31, 816-829 (2017)(PMID:28487407)
  4. Shen EZ et al. Identification of piRNA Binding Sites Reveals the Argonaute Regulatory Landscape of the C. elegans Germline. Cell.172, 937-951.e18 (2018)(PMID:29456082)
  5. Davidson L et al. Rapid Depletion of DIS3, EXOSC10, or XRN2 Reveals the Immediate Impact of Exoribonucleolysis on Nuclear RNA Metabolism and Transcriptional Control. Cell Rep. 26, 2779-2791.e5 (2019)(PMID:30840897)
  6. Yesbolatova A et al. The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat Commun. 11, 5701 (2020) (PMID:33177522)
References
  1. Nishimura K and Kanemaki MT et al. Rapid depletion of budding yeast proteins via the fusion of an Auxin-Inducible Degron (AID). Curr Protoc Cell Biol. 64, 20.9.1-20.9.16 (2014)（PMID: 25181302）
  2. Kubota T et al. The Elg1 replication factor C-like complex fuctions in PCNA unloading during DNA replication. Mol Cell. 50, 273-80 (2013)（PMID: 23499004）
  3. Nishimura K et al. An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nat Methods. 6, 917-22 (2009)（PMID: 19915560）
Product category
Tools
Epitope tags
この製品に関するお問い合わせをする

  • The availability is based on the information in Japan at 00:05, Apr 17, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.