Anti-GABARAP mAb

Availability (in Japan)

10 or more

（In Japan at 00:05,
Apr 20, 2024 in JST）

Size

100 µL (1 mg/mL)

Citations

Data Sheet

SDS

Data	Western Blotting
Clonality	Monoclonal	Clone	1F4
Isotype (Immunized Animal)	Mouse IgG1
Applications	WB 1 µg/mL IC* reported. （PMID: 22948227） IH* reported. （PMID: 21684337）
Immunogen (Antigen)	Carrier protein conjugated human GABARAP N-terminal synthetic peptide
Reactivity [Gene ID]	Human[11337], Mouse[56486], Rat[58974], Hamster[100767236], Chicken*[107050619](PMID: 25514832)
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	GABA(A) receptor-associated protein, MM46, ATG8A, GABARAP-a
Background	Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. LC3, GABARAP (γ-aminobutyric-acid-type-A-receptor-associated protein), and GATE-16 (Golgi-associated ATPase enhancer of 16 kDa) have been identified as a homologue of yeast Atg8. These homologues have been characterized as modifiers in reactions mediated by hAtg7 (an E1-like enzyme) and hAtg3 (an E2-like enzyme) as in yeast Atg8 lipidation. These homologues also generate form II, which are recovered in membrane fractions. Generation of the form II correlates with autophagosome association. These results suggest that all mammalian Atg8 homologues receive a common modification to associate with autophagosomal membrane as the form II.
Related products	PM037 Anti-GABARAP pAb PM038 Anti-GATE-16 pAb PM072 Anti-VMP1 pAb PM036 Anti-LC3 pAb M152-3 Anti-LC3 mAb M186-3 Anti-LC3 mAb PD014 Anti-LC3 pAb PM045 Anti-p62 (SQSTM1) pAb M162-3 Anti-p62 (SQSTM1) (Human) mAb PM066 Anti-p62 C-terminal pAb M133-3 Anti-Atg3 mAb PM039 Anti-Atg7 (Human) pAb PM090 Anti-Atg8 (Filamentous fungi) pAb M152-3MS Anti-LC3 mAb for My select sampler set
Citations	Western Blotting Tanji K et al. Alteration of autophagosomal proteins (LC3, GABARAP and GATE-16) in Lewy body disease. Neurobiol Dis. 43, 690-7 (2011)(PMID:21684337) Zhang Z et al. Gene deletion of Gabarap enhances Nlrp3 inflammasome-dependent inflammatory responses. J Immunol.190, 3517-24 (2013)(PMID:23427251) Maynard S et al. GABARAP is a determinant of apoptosis in growth-arrested chicken embryo fibroblasts. J Cell Physiol. 230, 1475-88 (2015)(PMID:25514832) Hervás JH et al. Human ATG3 binding to lipid bilayers: role of lipid geometry, and electric charge. Sci Rep. 7, 15614 (2017)(PMID:29142222) Immunoprecipitation Joachim J et al. Centriolar Satellites Control GABARAP Ubiquitination and GABARAP-Mediated Autophagy. Curr Biol. 27, 2123-2136.e7 (2017)(PMID:28712572) Immunocytochemistry Colecchia D et al. MAPK15/ERK8 stimulates autophagy by interacting with LC3 and GABARAP proteins. Autophagy 8, 1724-40 (2012)22948227(PMID:22948227) Immunohistochemistry Tanji K et al. Alteration of autophagosomal proteins (LC3, GABARAP and GATE-16) in Lewy body disease. Neurobiol Dis. 43, 690-7 (2011)(PMID:21684337)
Product category	Research area Autophagy

The availability is based on the information in Japan at 00:05, Apr 20, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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