Anti-Atg3 mAb for My select sampler set

Availability (in Japan)

1-9

（In Japan at 00:05,
Jan 19, 2021 in JST）

Size

20 µg/20 µL

Data	Western Blotting Immunoprecipitation
Clonality	Monoclonal	Clone	3E8
Isotype (Immunized Animal)	Mouse IgG2bκ
Applications	WB 1 μg/mL IP 2.5 µg/300 µL of cell extract from 3x10⁶ cells IC 0.5 μg/mL
Immunogen (Antigen)	Recombinant Human Atg3
Reactivity [Gene ID]	Human[64422], Mouse[67841], Rat[171415], Hamster
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	autophagy related 3, ATG3, APG3, APG3L, PC3-96, APG3-LIKE
Citations	Western Blotting Otomo C et al. Structure of the human ATG12~ATG5 conjugate required for LC3 lipidation in autophagy. Nat Struct Mol Biol. 20, 59-66 (2013)(PMID:23202584) Metlagel Z et al. Structural basis of ATG3 recognition by the autophagic ubiquitin-like protein ATG12. PNAS. 110, 18844-9 (2013)(PMID:24191030) Choi J et al. The parasitophorous vacuole membrane of Toxoplasma gondii is targeted for disruption by ubiquitin-like conjugation systems of autophagy. Immunity. 40, 924-35 (2014)(PMID:24931121) Yamashita SI et al. Mitochondrial division occurs concurrently with autophagosome formation but independently of Drp1 during mitophagy. J Cell Biol. 215, 649-665 (2016)(PMID:27903607) Allavena G et al. Suppressed translation and ULK1 degradation as potential mechanisms of autophagy limitation under prolonged starvation. Autophagy 12, 2085–2097 (2016)(PMID:27629431)
Product category	Research area Autophagy Cellular stress

The availability is based on the information in Japan at 00:05, Jan 19, 2021 in JST.
The special price is shown in red color.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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