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HOME > Product search results > Code No. M132-3 Anti-HA-tag mAb

Code No.　M132-3

Anti-HA-tag mAb

Availability (in Japan)

10 or more

（In Japan at 17:00,
Apr 19, 2024 in JST）

Size

100 µL (2 mg/mL)

Data

  • Western Blotting

  • Immunoprecipitation
Clonality Monoclonal Clone 5D8
Isotype (Immunized Animal) Mouse IgG1 κ
Applications
WB
1 µg/mL  
IP
1 µg/sample  
Co-IP*
reported.  （PMID: 20506502 / 21586271 / 27697867）
Immunogen (Antigen) Carrier protein conjugated peptide (CP-YPYDVPDYA)
Storage buffer 2 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Background Epitope tagging has widely been accepted technique that fuses an epitope peptide to a certain protein as a marker for gene expression. With this technique, the gene expression can be easily monitored on western blotting, immunoprecipitation and immunofluorescence utilizing with an antibody that recognizes such an epitope. Amino acid sequences that are widely used for the epitope tagging are as follow; YPYDVPDYA (HA-tag), EQKLISEEDL (Myc-tag) and YTDIEMNRLGK (VSV-G-tag), which corresponding to the partial peptide of Influenza hemagglutinin protein, Human c-myc gene product and Vesicular stomatitis virus glycoprotein respectively.
Related products M132-10 Anti-HA-tag mAb-Magnetic Agarose
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M180-10 Anti-HA-tag mAb-Magnetic Agarose
M180-A48 Anti-HA-tag mAb-Alexa Fluor™ 488
M180-A59 Anti-HA-tag mAb-Alexa Fluor™ 594
M180-A64 Anti-HA-tag mAb-Alexa Fluor™ 647
561 Anti-HA-tag pAb
561-5 Anti-HA-tag pAb
561-8 Anti-HA-tag pAb-Agarose
M132-11 Anti-HA-tag mAb-Magnetic Beads
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Citations

Western Blotting

  1. Ohshima T et al. HTLV‐1 basic leucine‐zipper factor, HBZ, interacts with MafB and suppresses transcription through a Maf recognition element. J Cell Biochem. 111, 187-94 (2010)(PMID:20506502)
  2. Kim TS et al. The ZFHX3 (ATBF1) transcription factor induces PDGFRB, which activates ATM in the cytoplasm to protect cerebellar neurons from oxidative stress. Dis Model Mech. 3, 752-62 (2010)(PMID:20876357)
  3. Mukai R, Ohshima T. Dual effects of HTLV-1 bZIP factor in suppression of interferon regulatory factor 1. Biochem Biophys Res Commun. 409, 328-32 (2011)(PMID:21586271)
  4. Mukai R, Ohshima T. HTLV-1 HBZ positively regulates the mTOR signaling pathway via inhibition of GADD34 activity in the cytoplasm. Oncogene. 33, 2317-28 (2014)(PMID:23708656)
  5. Zhang HH et al. Nemo-like kinase is critical for p53 stabilization and function in response to DNA damage. Cell Death Differ. 21, 1656-63 (2014)(PMID:24926618)
  6. Mukai R, Ohshima T. Enhanced Stabilization of MCL1 by the Human T-Cell Leukemia Virus Type 1 bZIP Factor Is Modulated by Blocking the Recruitment of Cullin 1 to the SCF Complex. Mol Cell Biol. 36, 3075-3085 (2016)(PMID:27697867)
  7. Kitao T et al. Legionella Manipulates Non-canonical SNARE Pairing Using a Bacterial Deubiquitinase. Cell Rep. 32, 108107 (2020) (PMID:32905772)

Co-IP

  1. Ohshima T et al. HTLV‐1 basic leucine‐zipper factor, HBZ, interacts with MafB and suppresses transcription through a Maf recognition element. J Cell Biochem. 111, 187-94 (2010)(PMID:20506502)
  2. Mukai R, Ohshima T. Dual effects of HTLV-1 bZIP factor in suppression of interferon regulatory factor 1. Biochem Biophys Res Commun. 409, 328-32 (2011)(PMID:21586271)
  3. Mukai R, Ohshima T. Enhanced Stabilization of MCL1 by the Human T-Cell Leukemia Virus Type 1 bZIP Factor Is Modulated by Blocking the Recruitment of Cullin 1 to the SCF Complex. Mol Cell Biol. 36, 3075-3085 (2016)(PMID:27697867)

Immunoprecipitation

  1. Ogi T et al. Identification of the first ATRIP-deficient patient and novel mutations in ATR define a clinical spectrum for ATR-ATRIP Seckel Syndrome. PLoS Genet. 8, e1002945 (2012)(PMID:23144622)
  2. Mukai R, Ohshima T. HTLV-1 HBZ positively regulates the mTOR signaling pathway via inhibition of GADD34 activity in the cytoplasm. Oncogene. 33, 2317-28 (2014)(PMID:23708656)
  3. Zhang HH et al. Nemo-like kinase is critical for p53 stabilization and function in response to DNA damage. Cell Death Differ. 21, 1656-63 (2014)(PMID:24926618)

Immunocytochemistry

  1. Kitao T et al. Legionella Manipulates Non-canonical SNARE Pairing Using a Bacterial Deubiquitinase. Cell Rep. 32, 108107 (2020) (PMID:32905772)
Product category
Tools
Epitope tags
この製品に関するお問い合わせをする

  • The availability is based on the information in Japan at 17:00, Apr 19, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.