This site is for customers in Asia. Customers in China & other regions, please go to Global page.
HOME > Product search results > Code No. M047-3 Anti-Myc-tag mAb

Code No.　M047-3

Anti-Myc-tag mAb

Availability (in Japan)

10 or more

（In Japan at 00:05,
Apr 20, 2024 in JST）

Size

100 µL (2 mg/mL)

Data

  • Western Blotting
Clonality Monoclonal Clone PL14
Isotype (Immunized Animal) Mouse IgG1 κ
Applications
WB
1 µg/mL  
IP
5 µg/sample  
IC
2 µg/mL  
RIP*
reported.  （PMID: 23064114 / 26738979）
ChIP*
reported.  （PMID: 19763945）
Immunogen (Antigen) 6myc-tag fusion protein
Storage buffer 2 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Background Epitope tagging has widely been accepted technique that fuses an epitope peptide to a certain protein as a marker for gene expression. With this technique, the gene expression can be easily monitored on western blotting, immunoprecipitation and immunofluorescence utilizing with an antibody that recognizes such an epitope. Amino acid sequences that are widely used for the epitope tagging are as follow; YPYDVPDYA (HA-tag), EQKLISEEDL (Myc-tag) and YTDIEMNRLGK (VSV-G-tag), which corresponding to the partial peptide of Influenza hemagglutinin protein, human c-myc gene product and Vesicular stomatitis virus glycoprotein respectively.
Related products M047-8 Anti-Myc-tag mAb-Agarose
M047-11 Anti-Myc-tag mAb-Magnetic Beads
M047-10 Anti-Myc-tag mAb-Magnetic Agarose
M047-A48 Anti-Myc-tag mAb-Alexa Fluor™ 488
M047-A59 Anti-Myc-tag mAb-Alexa Fluor™ 594
M047-A64 Anti-Myc-tag mAb-Alexa Fluor™ 647
M192-3 Anti-Myc-tag mAb
M192-7 Anti-Myc-tag mAb-HRP-DirecT
562 Anti-Myc-tag pAb
562-5 Anti-Myc-tag pAb
Citations

Western Blotting

  1. Ren J et al. Methylation of ribosomal protein S10 by protein-arginine methyltransferase 5 regulates ribosome biogenesis. J Biol Chem. 285,12695-705 (2010)(PMID:20159986)
  2. Sun L et al. Substrate phosphorylation and feedback regulation in JFK-promoted p53 destabilization. J Biol Chem. 286, 4226-35 (2011)(PMID:21127074)
  3. Sato T et al. Identification of 14-3-3 proteins as a target of ATL31 ubiquitin ligase, a regulator of the C/N response in Arabidopsis. Plant J. 68,137-46 (2011)(PMID:21668537)
  4. Fang G et al. Centlein mediates an interaction between C-Nap1 and Cep68 to maintain centrosome cohesion. J Cell Sci. 127, 1631-9 (2014)(PMID:24554434)
  5. Chen X et al. Coronavirus membrane-associated papain-like proteases induce autophagy through interacting with Beclin1 to negatively regulate antiviral innate immunity. Protein Cell. 5, 912-27 (2014)(PMID:25311841)
  6. Shin C et al. MKRN2 is a novel ubiquitin E3 ligase for the p65 subunit of NF-κB and negatively regulates inflammatory responses. Sci Rep. 7, 46097 (2017)(PMID:28378844)
  7. Huang Y et al. RLIM suppresses hepatocellular carcinogenesis by up-regulating p15 and p21. Oncotarget. 8, 83075-83087 (2017)(PMID:29137325)
  8. Yang S et al. USP52 acts as a deubiquitinase and promotes histone chaperone ASF1A stabilization. Nat Commun. 9, 1285 (2018)(PMID:29599486)
  9. Chen Y et al. Mutually exclusive acetylation and ubiquitylation of the splicing factor SRSF5 control tumor growth. Nat Commun. 9, 2464 (2018)(PMID:29942010)
  10. Yang L et al. Reconstituting Arabidopsis CRY2 Signaling Pathway in Mammalian Cells Reveals Regulation of Transcription by Direct Binding of CRY2 to DNA. Cell Rep. 24, 585-593.e4(2018)(PMID:30021157)
  11. Dansako H et al. High‐level expression of STING restricts susceptibility to HBV by mediating type III IFN induction. FASEB BioAdvances 2018, 1-14 (2018)
  12. Fujibayashi E et al. Isolation of cancer cells with augmented spheroid-forming capability using a novel tool equipped with removable filter. Oncotarget 9, 33931-33946 (2018)(PMID:30338036)
  13. Cui CP et al. Dynamic ubiquitylation of Sox2 regulates proteostasis and governs neural progenitor cell differentiation. Nat Commun. 9, 4648 (2018)(PMID:30405104)
  14. Yamamoto T et al. DUSP10 constrains innate IL-33-mediated cytokine production in ST2hi memory-type pathogenic Th2 cells. Nat Commun. 9, 4231 (2018)(PMID:30315197)
  15. Ariumi Y et al. DNA repair protein Rad18 restricts LINE-1 mobility. Sci Rep. 8, 15894 (2018)(PMID:30367120)
  16. Gao T et al. PKCζ Phosphorylates SIRT6 to Mediate Fatty Acid β-Oxidation in Colon Cancer Cells. Neoplasia. 21, 61-73 (2019) (PMID:30504065)
  17. Cui CP et al. Author Correction: Dynamic ubiquitylation of Sox2 regulates proteostasis and governs neural progenitor cell differentiation. Nat Commun. 10, 173 (2019)(PMID:30622263)
  18. Wu R et al. A novel m6A reader Prrc2a controls oligodendroglial specification and myelination. Cell Res. 29, 23-41 (2019) (PMID:30514900)
  19. Fan J et al. CKIP-1 limits foam cell formation and inhibits atherosclerosis by promoting degradation of Oct-1 by REGγ. Nat Commun. 10, 425 (2019)(PMID:30683852)
  20. Xu R et al. hCINAP regulates the DNA-damage response and mediates the resistance of acute myelocytic leukemia cells to therapy. Nat Commun. 10, 3812 (2019)(PMID:31444354)
  21. Lu X et al. GLP-catalyzed H4K16me1 promotes 53BP1 recruitment to permit DNA damage repair and cell survival. Nucleic Acids Res. 47, 10977-10993 (2019)(PMID:31612207)
  22. Lu YF et al. SIRT7 activates p53 by enhancing PCAF-mediated MDM2 degradation to arrest the cell. Oncogene. 39, 4650-4665 (2020)(PMID:32404984)
  23. Kong X et al. Hematopoietic Cell Kinase (HCK) Is Essential for NLRP3 Inflammasome Activation and Lipopolysaccharide-Induced Inflammatory Response In Vivo. Front Pharmacol. 11, 581011 (2020)(PMID:33041826)

ChIP

  1. Bermejo R et al. ChIP-on-chip analysis of DNA topoisomerases. Methods Mol Biol. 582, 103-18 (2009)(PMID:19763945)

Immunoprecipitation

  1. Ren J et al. Methylation of ribosomal protein S10 by protein-arginine methyltransferase 5 regulates ribosome biogenesis. J Biol Chem. 285,12695-705 (2010)(PMID:20159986)
  2. Arii J et al. Non-muscle myosin IIA is a functional entry receptor for herpes simplex virus-1. Nature 467, 859-62 (2010)(PMID:20944748)
  3. Sun L et al. Substrate phosphorylation and feedback regulation in JFK-promoted p53 destabilization. J Biol Chem. 286, 4226-35 (2011)(PMID:21127074)
  4. Hirano Y et al. Structural basis of cargo recognition by the myosin-X MyTH4-FERM domain. EMBO J. 30, 2734-47 (2011)(PMID:21642953)
  5. Fang G et al. Centlein mediates an interaction between C-Nap1 and Cep68 to maintain centrosome cohesion. J Cell Sci. 127, 1631-9 (2014)(PMID:24554434)
  6. Chen X et al. Coronavirus membrane-associated papain-like proteases induce autophagy through interacting with Beclin1 to negatively regulate antiviral innate immunity. Protein Cell. 5, 912-27 (2014)(PMID:25311841)
  7. Huang Y et al. RLIM suppresses hepatocellular carcinogenesis by up-regulating p15 and p21. Oncotarget. 8, 83075-83087 (2017)(PMID:29137325)
  8. Chen Y et al. Mutually exclusive acetylation and ubiquitylation of the splicing factor SRSF5 control tumor growth. Nat Commun. 9, 2464 (2018)(PMID:29942010)
  9. Yang L et al. Reconstituting Arabidopsis CRY2 Signaling Pathway in Mammalian Cells Reveals Regulation of Transcription by Direct Binding of CRY2 to DNA. Cell Rep. 24, 585-593.e4(2018)(PMID:30021157)
  10. Cui CP et al. Dynamic ubiquitylation of Sox2 regulates proteostasis and governs neural progenitor cell differentiation. Nat Commun. 9, 4648 (2018)(PMID:30405104)
  11. Yamamoto T et al. DUSP10 constrains innate IL-33-mediated cytokine production in ST2hi memory-type pathogenic Th2 cells. Nat Commun. 9, 4231 (2018)(PMID:30315197)
  12. Ariumi Y et al. DNA repair protein Rad18 restricts LINE-1 mobility. Sci Rep. 8, 15894 (2018)(PMID:30367120)
  13. Cui CP et al. Author Correction: Dynamic ubiquitylation of Sox2 regulates proteostasis and governs neural progenitor cell differentiation. Nat Commun. 10, 173 (2019)(PMID:30622263)
  14. Lu X et al. GLP-catalyzed H4K16me1 promotes 53BP1 recruitment to permit DNA damage repair and cell survival. Nucleic Acids Res. 47, 10977-10993 (2019)(PMID:31612207)

Immunocytochemistry

  1. Senju Y et al. Essential role of PACSIN2/syndapin-II in caveolae membrane sculpting. J Cell Sci. 124, 2032-40 (2011)(PMID:21610094)
  2. Fang G et al. Centlein mediates an interaction between C-Nap1 and Cep68 to maintain centrosome cohesion. J Cell Sci. 127, 1631-9 (2014)(PMID:24554434)
  3. Shin C et al. MKRN2 is a novel ubiquitin E3 ligase for the p65 subunit of NF-κB and negatively regulates inflammatory responses. Sci Rep. 7, 46097 (2017)(PMID:28378844)

RNP Immunoprecipitation

  1. Tani H et al. Identification of hundreds of novel UPF1 target transcripts by direct determination of whole transcriptome stability. RNA Biol. 9, 1370-9 (2012)(PMID:23064114)
  2. Arimoto-Matsuzaki K  et al. TIA1 oxidation inhibits stress granule assembly and sensitizes cells to stress-induced apoptosis. Nat Commun. 7, 10252 (2016)(PMID:26738979)
Product category
Tools
Epitope tags
この製品に関するお問い合わせをする

  • The availability is based on the information in Japan at 00:05, Apr 20, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.