Anti-DNA Topoisomerase IIα (Human) mAb

Availability (in Japan)

10 or more

（In Japan at 00:05,
Apr 26, 2024 in JST）

Size

100 µL (1 mg/mL)

Citations

Data Sheet

SDS

Data	Western Blotting Flow Cytometry Immunocytochemistry Immunohistochemistry
Clonality	Monoclonal	Clone	1C5
Isotype (Immunized Animal)	Mouse IgG2a
Applications	WB 0.1-0.5 µg/mL IP 1-10 µg/200-300 µL of cell extract FCM 0.1-1 µg/mL (final concentration) IC 1-10 µg/mL IH 1-5 µg/mL (Heat treatment is necessary for paraffin embedded sections.)
Immunogen (Antigen)	Recombinant Human DNA Topo IIα (C-terminal 182 a.a.)
Reactivity [Gene ID]	Human[7153], Mouse(-), Rat(-), Hamster(-)
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	TOP2A, TOP2, TP2A
Background	Topoisomerase II (Topo II) is a nuclear enzyme that regulates the topological states of DNA by transient breakage and rejoining double-stranded DNA, catalyzing the decatenation and unknotting of topologically linked DNA circles and the relaxation of supercoiled DNA. In mammalian cells, Topo II consists of two isozymes, Topo IIα (170 kDa) and Topo IIβ (180 kDa). Expression and localization of each isoform are distinct and stage specific during the cell cycle. Topo IIβ is expressed constantly throughout cell cycle, whereas the expression of Topo IIα is cell cycle-regulated, peaking in G₂ to M phase and declining to a minimal level at the end of M phase. It is considered that Topo IIα plays an essential role in cell proliferation, especially during late S to M phase.
Citations	Western Blotting Antoniou-Kourounioti M et al. The Impact of the C-Terminal Region on the Interaction of Topoisomerase II Alpha with Mitotic Chromatin. 20, pii: E1238 (2019)(PMID:30871006) Yesbolatova A et al. The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat Commun. 11, 5701 (2020)(PMID:33177522) Immunohistochemistry Antoniou-Kourounioti M et al. The Impact of the C-Terminal Region on the Interaction of Topoisomerase II Alpha with Mitotic Chromatin. 20, pii: E1238 (2019)(PMID:30871006) Immunofluorescence Nagashima R et al. Single nucleosome imaging reveals loose genome chromatin networks via active RNA polymerase II. J Cell Biol. 218, 1511-1530 (2019) (PMID:30824489) Venegas AB et al. Inducible Degradation of the Human SMC5/6 Complex Reveals an Essential Role Only during Interphase. Cell Rep. 31, 107533 (2020)(PMID:32320646)
Product category	Research area Cancer

The availability is based on the information in Japan at 00:05, Apr 26, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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