Anti-BAX (Human) mAb

Availability (in Japan)

10 or more

（In Japan at 17:00,
Apr 19, 2024 in JST）

Size

100 µg

Citations

Data Sheet

SDS

Data	Western Blotting Immunoprecipitation Flow Cytometry Immunocytochemistry Immunohistochemistry
Clonality	Monoclonal	Clone	4F11
Isotype (Immunized Animal)	Mouse IgG2bκ
Applications	WB 0.1-1 µg/mL IP 5-10 µg /200 µL of cell extract from 5x10⁶ cells FCM 5-10 µg/mL (final concentration) IC 10 µg/mL IH 10 µg/mL (Heat treatment is necessary for paraffin embedded sections.)
Immunogen (Antigen)	Recombinant Human BAX protein
Reactivity [Gene ID]	Human[581], Mouse(-)
Storage buffer	lyophilized form. After reconstitution with 100 µL of distilled water, the IgG concentration should be 1mg/mL in PBS/1% sucrose, pH 7.2
Storage temp.	4°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	BCL2 associated X, apoptosis regulator
Background	Bax (Bcl-associated X protein) is a 21 kDa tumor suppressor protein that suppresses tumorigenesis and stimulates apoptosis in vivo. Bax has extensive amino acid homology to Bcl-2. It can homodimerize through its BH3 domain and it forms heterodimers with other Bcl-2 family members through its BH1 and BH2 domains. Overexpression of Bax promotes apoptosis and counters the death repressor activity of Bcl-2 and Bcl-xL. It is believed that the ratio of Bcl-2/Bax complexes to free protein controls the relative susceptibility of cells to death stimuli. Apoptotic stimuli cause the translocation of monomeric Bax from the cytosol to the mitochondria where it forms Bax homodimers. Localization of Bax to the mitochondria results in the activation of caspase-3, membrane blebbing, and nuclear fragmentation. Bax also induces mitochondrial dysfunction by increasing mitochondrial membrane permeability. It accelerates the opening of the mitochondrial porin channel VDAC, thus regulating the release of cytochrome c during apoptosis.
Related products	M160-3 Anti-UVRAG mAb
Citations	Western Blotting Wang Y et al. Deletion of INMAP postpones mitotic exit and induces apoptosis by disabling the formation of mitotic spindle. Biochem Biophys Res Commun. 518, 19-25 (2019)(PMID:31405563)
Product category	Research area Apoptosis

The availability is based on the information in Japan at 17:00, Apr 19, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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