Anti-HLA-E (Human) mAb

Availability (in Japan)

10 or more

（In Japan at 17:00,
Jan 25, 2025 in JST）

Size

100 µL (1 mg/mL)

Citations

Data Sheet

SDS

Data	Flow Cytometry
Clonality	Monoclonal	Clone	4D12
Isotype (Immunized Animal)	Mouse IgG1
Applications	FCM 10 µg/mL (final concentration) IH* reported. （PMID: 20947660）
Immunogen (Antigen)	This antibody was established by immunization of HLA-B27 transgenic mice with HLA-E protein.
Reactivity [Gene ID]	Human
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Background	HLA-E (human leucocyte antigen-E) is a conserved class I major histocompatibility molecule. It binds to the leader peptide derived from the polymorphic classical MHC molecules HLA-A, HLA-B and HLA-C. This peptide binding stabilizes the HLA-E protein and allows it to migrate to the cell surface. HLA-E then interacts with CD94/NKG2A receptors on natural killer cells. This interaction inhibits natural killer cell-mediated lysis of cells displaying HLA-E. In virally infected or tumor cells, down-regulation of HLA-A, HLA-B and HLA-C production prevents stabilization of HLA-E by the leader peptide. Under these circumstances, HLA-E is degraded before it reaches the cell surface and the cell is then vulnerable to lysis by natural killer cells.
Related products	K0126-3 Anti-HLA-E (Human) mAb K0208-3 Anti-HLA-A24 (Human) mAb K0208-4 Anti-HLA-A24 (Human) mAb-FITC K0208-5 Anti-HLA-A24 (Human) mAb-PE K0208-A48 Anti-HLA-A24 (Human) mAb-Alexa Fluor™ 488 K0209-3 Anti-HLA-A24 (Human) mAb K0209-4 Anti-HLA-A24 (Human) mAb-FITC K0209-5 Anti-HLA-A24 (Human) mAb-PE
Citations	Flow Cytometry Nguyen S et al. HLA-E upregulation on IFN-gamma-activated AML blasts impairs CD94/NKG2A-dependent NK cytolysis after haplo-mismatched hematopoietic SCT. Bone Marrow Transplant. 43, 693-9 (2009)(PMID:19011664) Tsukamoto N et al. Impairment of plasmacytoid dendritic cells for IFN production by the ligand for immunoglobulin-like transcript 7 expressed on human cancer cells. Clin Cancer Res. 15, 5733-43 (2009)(PMID:19723650) Immunohistochemistry Cui CH et al. Dystrophin conferral using human endothelium expressing HLA-E in the non-immunosuppressive murine model of Duchenne muscular dystrophy. Hum Mol Genet. 20, 235-44 (2011)(PMID:20947660)
Product category	Research area Immunology Cell surface antigens

The availability is based on the information in Japan at 17:00, Jan 25, 2025 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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