Data |
Western Blotting
 Data were kindly provided from Dr. Yoshiaki Ito and Dr. Kosei Ito. (Institute of Molecular and Cell Biology, Singapore)
Immunohistochemistry
 Data were kindly provided from Dr. Yoshiaki Ito and Dr. Kosei Ito. (Institute of Molecular and Cell Biology, Singapore)
|
Clonality |
Monoclonal
|
Clone |
R3-6E9 |
Isotype
(Immunized Animal)
|
Mouse IgG1
|
Applications |
- WB
- 0.2-1 µg/mL
- IH
- 2 µg/mL (Heat treatment is necessary for paraffin embedded sections.)

|
Immunogen
(Antigen) |
Human RUNX3 polypeptide
|
Reactivity
[Gene ID] |
Human[864], Mouse[12399] |
Storage buffer |
1 mg/mL in PBS/50% glycerol, pH 7.2
|
Storage temp. |
-20°C
|
Conjugate |
Unlabeled |
Manufacturer |
MBL |
Alternative names |
RP3-398I9.1, AML2, CBFA3, PEBP2aC
|
Background |
The Runx (runt-related protein) family of transcription factors plays important roles in different tissues and cell lineages. Runx1 determines commitment to the hematopoietic cell lineage and Runx2 determines commitment to the osteoblastic lineage. Runx3 is involved in gastric epithelial growth and differentiation. PEBP2/Cbfβ is required for Runx-dependent transcriptional regulation. Runx proteins interact with many other transcription factors and co-regulators in the transcriptional regulation of their target genes. |
Related products |
D235-3 Anti-RUNX3 mAb D236-3 Anti-RUNX3 mAb D207-3 Anti-Runx mAb D208-3 Anti-Runx mAb D130-3 Anti-Runx2 (Cbfa1) mAb D127-3 Anti-PEBP2β (CBFβ) (Human) mAb
|
Citations |
ChIP - Chang TL et al Claudin-1 has tumor suppressive activity and is a direct target of RUNX3 in gastric epithelial cells. Gastroenterology 138, 255-265 (2010)(PMID:19706291)
- Presnell SR et al. Differential transcription factor use by the KIR2DL4 promoter under constitutive and IL-2/15-treated conditions. J Immunol. 188, 4394-404 (2012)(PMID:22467658)
- Hor YT et al.A role for RUNX3 in inflammation-induced expression of IL23A in gastric epithelial cells. Cell Rep. 8, 50-8 (2014)(PMID:25008775)
Immunoprecipitation - Qiao Y et al. RUNX3 is a novel negative regulator of oncogenic TEAD-YAP complex in gastric cancer. Oncogene 35, 2664-74 (2016)(PMID:26364597)
Immunohistochemistry - Chang TL et al Claudin-1 has tumor suppressive activity and is a direct target of RUNX3 in gastric epithelial cells. Gastroenterology 138, 255-265 (2010)(PMID:19706291)
- Lin FC et al. RUNX3-mediated transcriptional inhibition of Akt suppresses tumorigenesis of human gastric cancer cells. Oncogene 31, 4302-16 (2012)(PMID:22231444)
|
Product category |
- Research area
- Stem cell research
Cancer
|