Anti-CD274 (PD-L1) (Human) mAb

Availability (in Japan)

10 or more

（In Japan at 17:00,
Oct 8, 2024 in JST）

Size

100 µL (1 mg/mL)

Citations

Data Sheet

SDS

Data	Flow Cytometry
Clonality	Monoclonal	Clone	27A2
Isotype (Immunized Animal)	Mouse IgG2b
Applications	FCM 10 µg/mL (final concentration) IH 10 µg/mL (Heat treatment is necessary for paraffin embedded sections.)
Immunogen (Antigen)	Recombinant Human PD-L1 extracellular domain
Reactivity [Gene ID]	Human[29126]
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	B7H1
Background	Programmed death ligand 1 (PD-L1, also known as CD274/B7-H1), a member of B7 family was identified by searching for molecules that share homology with the immunogloblin V and C domains of B7-1 and B7-2 among the human cDNA expressed sequence tags in the National Center for Biotechnology Information database. PD-L1 is a ligand for programmed death 1 (PD-1) which belongs to the CD28/CTLA4 subfamily. Although <em>in vitro</em> study indicated that the cross-linking of PD-1 by PD-L1 leads to down-regulation of T-cell responses, some studies have shown that T cells stimulated with low levels of anti-CD3 and immobilized PD-L1-Ig were activated, proliferation and production of IFN-γ GM-CSF and IL-10 from the T cells were enhanced. The role of PD-L1 is now debatable.
Related products	D230-5 Anti-CD274 (PD-L1) (Human) mAb-PE D092-3M2 Anti-CD274 (PD-L1) (Human) mAb (Functional Grade) D133-3M2 Anti-CD279 (PD-1) (Human) mAb (Functional Grade) D092-3 Anti-CD274 (PD-L1) (Human) mAb D132-3 Anti-CD279 (PD-1) (Human) mAb D132-4 Anti-CD279 (PD-1) (Human) mAb-FITC D132-5 Anti-CD279 (PD-1) (Human) mAb-PE D133-5 Anti-CD279 (PD-1) (Human) mAb-PE
Citations	Immunohistochemistry Hamanishi J et al. Programmed cell death 1 ligand 1 and tumor-infiltrating CD8+ T lymphocytes are prognostic factors of human ovarian cancer. PNAS. 104, 3360-5 (2007)(PMID:1736065) Schuchmann M et al. The programmed death (PD)-1/PD-ligand 1 pathway regulates graft-versus-host-reactive CD8 T cells after liver transplantation. Am J Transplant. 8, 2434-44 (2008)(PMID:18925909) Hino R et al. Tumor cell expression of programmed cell death-1 ligand 1 is a prognostic factor for malignant melanoma. Cancer 116, 1757-66 (2010)(PMID:20143437) Gadiot J et al. Overall survival and PD-L1 expression in metastasized malignant melanoma. Cancer 117, 2192-201 (2011)(PMID:21523733) Droeser RA et al. Clinical impact of programmed cell death ligand 1 expression in colorectal cancer. Eur J Cancer 49, 2233-42 (2013)(PMID:23478000) Hamanishi J et al. Safety and Antitumor Activity of Anti-PD-1 Antibody, Nivolumab, in Patients With Platinum-Resistant Ovarian Cancer. J Clin Oncol. 33, 4015-22 (2015)(PMID:26351349) Wu X et al. PD-1(+) CD8(+) T cells are exhausted in tumours and functional in draining lymph nodes of colorectal cancer patients. Br J Cancer. 111, 1391-9 (2014)(PMID:25093496) Goto W et al. Eribulin Promotes Antitumor Immune Responses in Patients with Locally Advanced or Metastatic Breast Cancer. Anticancer Res. 38, 2929-2938 (2018)(PMID:29715119) Ito N et al. Clinical Significance of Programmed Death Ligand-1 Expression in Esophageal Squamous Cell Carcinoma. J Surg Res. 251, 321-328 (2020)(PMID:32200323)
Product category	Research area Immunology Cancer Apoptosis Cell surface antigens

The availability is based on the information in Japan at 17:00, Oct 8, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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