Anti-MFG-E8 (Mouse) mAb

Availability (in Japan)

1-9

（In Japan at 00:05,
Apr 24, 2024 in JST）

Size

100 µL (1 mg/mL)

Citations

Data Sheet

SDS

Data	Western Blotting
Clonality	Monoclonal	Clone	18A2-G10
Isotype (Immunized Animal)	Hamster IgG
Applications	WB 1 µg/mL IH 1 µg/mL (Can be used in cryosections.)
Immunogen (Antigen)	Recombinant Mouse MFG-E8
Reactivity [Gene ID]	Mouse[17304]
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	P47, MP47, Mfgm, SED1, lactadherin
Background	Apoptotic cells are rapidly removed by phagocytosis to clear the living cells and tissues of intracellular materials being released from dying cells. Phagocytes recognize apoptotic cells through milk fat globule-EGF-factor 8 (MFG-E8). MFG-E8 is a secreted glycoprotein from thioglycollate-elicited macrophages and binds to phosphatidylserine exposed on the cell surface of apoptotic cells, then binds strongly to cells expressing avb3 integrin via its RGD (arginine-glycine-aspartate) motif. Thus, MFG-E8 is an important protein to mediate the engulfment of apoptotic cells by activated macrophages.
Related products	D161-3 Anti-MFG-E8 (Mouse) mAb
Citations	Western Blotting Aziz MM et al. Prolactin induces MFG-E8 production in macrophages via transcription factor C/EBPbeta-dependent pathway. Apoptosis. 13, 609-20 (2008)(PMID:18392683) Hoffhines AJ et al. Tyrosylprotein sulfotransferase-2 expression is required for sulfation of RNase 9 and Mfge8 in vivo. J Biol Chem. 284, 3096-105 (2009)(PMID:19047058) Bobrie A et al. Diverse subpopulations of vesicles secreted by different intracellular mechanisms are present in exosome preparations obtained by differential ultracentrifugation. J Extracell Vesicles. 1, 18397 (2012)(PMID:24009879) Aziz M et al. Milk fat globule-epidermal growth factor-factor 8 attenuates neutrophil infiltration in acute lung injury via modulation of CXCR2. J immunol. 189, 393-402 (2012)(PMID:22634615) Neutralization Fujiwara et al. The significance of tumor cells-derived MFG-E8 in tumor growth of angiosarcoma. J Dermatol Sci. 96, 18-25 (2019)(PMID:31447183) Immunohistochemistry Jinushi M et al. MFG-E8-mediated uptake of apoptotic cells by APCs links the pro- and antiinflammatory activities of GM-CSF. J Clin Invest. 117, 1902-13 (2007)(PMID:17557120) Kranich J et al. Follicular dendritic cells control engulfment of apoptotic bodies by secreting Mfge8. J Exp Med. 205, 1293-302 (2008)(PMID:18490487) Middendorp S et al. Mice deficient for CD137 ligand are predisposed to develop germinal center-derived B-cell lymphoma. Blood. 114, 2280-9 (2009)(PMID:19608748) Yoshino M et al. Analysis of capturing skin antigens in the steady state using milk fat globule EGF factor 8-deficient skin-hyperpigmented mice. Immunol Lett. 115, 131-37 (2008)(PMID:18083239) Abe T et al. Regulation of osteoclast homeostasis and inflammatory bone loss by MFG-E8. J Immunol. 193, 1383-91 (2014)(PMID:24958900)
Product category	Research area Apoptosis Exosomes

The availability is based on the information in Japan at 00:05, Apr 24, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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