Data |
Western Blotting
|
Clonality |
Monoclonal
|
Clone |
11B |
Isotype
(Immunized Animal)
|
Mouse IgG1 κ
|
Applications |
- WB
- 1 μg/mL
- IP*
- reported. (PMID: 12011038 / 12730204)
- IC*
- reported. (PMID: 19686243)
- IH*
- reported. (PMID: 17476974)
|
Immunogen
(Antigen) |
Human NEU3 transfected COS cells
|
Reactivity
[Gene ID] |
Human[10825] |
Storage buffer |
1 mg/mL in PBS/50% glycerol, pH 7.2
|
Storage temp. |
-20°C
|
Conjugate |
Unlabeled |
Manufacturer |
MBL |
Alternative names |
SIAL3
|
Background |
Gangliosides, sialic acid-containing glycosphingolipids, are present in surface membranes of cells and play important functional roles in regulating a wide range of biological processes including cell surface interactions, cell differentiation, and transmembrane signaling. Plasma membrane-associated ganglioside sialidase (NEU3) has been suggested to play essential roles in regulation of cell surface functions because of its major localization in the plasma membrane and strict substrate preference for gangliosides, thought to play important roles in cell surface events through modulation of gangliosides. |
Citations |
Western Blotting - Wang Y et al. A close association of the ganglioside-specific sialidase Neu3 with caveolin in membrane microdomains. J Biol Chem. 277, 26252-9 (2002)(PMID:12011038)
- Wada T et al. A crucial role of plasma membrane-associated sialidase in the survival of human cancer cells. Oncogene 26, 2483-90 (2007)(PMID:17334392)
- Gadhoum SZ, Sackstein R. CD15 expression in human myeloid cell differentiation is regulated by sialidase activity. Nat Chem Biol. 4, 751-7 (2008)(PMID:18953356)
- Wang J et al. Sialidase occurs in both membranes of the nuclear envelope and hydrolyzes endogenous GD1a. J Neurochem. 111, 547-54 (2009)(PMID:19686243)
Immunoprecipitation - Wang Y et al. A close association of the ganglioside-specific sialidase Neu3 with caveolin in membrane microdomains. J Biol Chem. 277, 26252-9 (2002)(PMID:12011038)
- Sasaki A et al. Overexpression of plasma membrane-associated sialidase attenuates insulin signaling in transgenic mice. J Biol Chem. 278, 27896-902 (2003)(PMID:12730204)
Immunocytochemistry - Wang J et al. Sialidase occurs in both membranes of the nuclear envelope and hydrolyzes endogenous GD1a. J Neurochem. 111, 547-54 (2009)(PMID:19686243)
Immunohistochemistry - Nomura H et al. Expression of NEU3 (plasma membrane-associated sialidase) in clear cell adenocarcinoma of the ovary: its relationship with T factor of pTNM classification. Oncol Res. 16, 289-97 (2006)(PMID:17476974)
|
Product category |
- Research area
- Cancer
Signal transduction
|