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HOME > Product search results > Code No. D153-3 Anti-GFP (Green Fluorescent Protein) mAb

Code No.　D153-3

Anti-GFP (Green Fluorescent Protein) mAb

Availability (in Japan)

1-9

（In Japan at 00:05,
Jan 23, 2025 in JST）

Size

100 µL (1 mg/mL)

Data

  • Immunoprecipitation

  • Immunocytochemistry
Clonality Monoclonal Clone RQ2
Isotype (Immunized Animal) Rat IgG2aκ
Applications
IP
0.5-2 µg/sample  
IC
2 µg/mL  
Immunogen (Antigen) GFP purified from GFP expressed 293T cells by affinity chromatographic technique using mouse anti-GFP
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Background Since the detection of intracellular Aequorea Victria Green Fluorescent Protein (GFP) requires only irradiation by UV or blue light, it provides an excellent means for monitoring gene expression and protein localization in living cells. Anti-GFP (Green Fluorescent Protein) mAb (clone RQ2) can detect GFP fusion protein on Immunoprecipitation and immunocytochemistry.
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Citations

Western Blotting

  1. Zhou H et al. Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2. Nat Commun. 7, 10151 (2016)(PMID:26743940)
  2. Nishimura R et al. Solo and Keratin Filaments Regulate Epithelial Tubule Morphology. Cell Struct Funct. 43, 95-105 (2018)(PMID:29709890)
  3. Li M et al. p53 cooperates with SIRT6 to regulate cardiolipin de novo biosynthesis. Cell Death Dis. 9, 941 (2018)(PMID:30237540)

Co-IP

  1. Qi Q et al. Involvement of the N-terminal B-box domain of Arabidopsis BBX32 protein in interaction with soybean BBX62 protein. J Biol Chem. 287, 31482-93 (2012)(PMID:22801428)

Immunoprecipitation

  1. Obuse C et al. A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1. Nat Cell Biol. 6, 1135-41 (2004)(PMID:15502821)
  2. Sakurai T et al. Membrane microdomain switching: a regulatory mechanism of amyloid precursor protein processing. J Cell Biol. 183, 339-52 (2008)(PMID:18936252)
  3. Sugiyama T et al. Red5 and three nuclear pore components are essential for efficient suppression of specific mRNAs during vegetative growth of fission yeast. Nucleic Acids Res. 41, 6674-86 (2013)(PMID:23658229)
  4. Zhou H et al. Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2. Nat Commun. 7, 10151 (2016)(PMID:26743940)
  5. Luteijn RD et al. Cowpox virus protein CPXV012 eludes CTLs by blocking ATP binding to TAP. J Immunol. 193, 1578-89 (2014)(PMID:25024387)
  6. Hatakeyama H et al. Cooperative actions of Tbc1d1 and AS160/Tbc1d4 in GLUT4-trafficking activities. J Biol Chem. 294, 1161-1172 (2019)(PMID:30482843)

Immunocytochemistry

  1. Kunishima S et al. ACTN1 mutations cause congenital macrothrombocytopenia. Am J Hum Genet. 92, 431-8 (2013)(PMID:23434115)
  2. Yamanaka T et al. Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation. Sci Rep. 10, 7610 (2020)(PMID:32376919)

Immunohistochemistry

  1. Matsuoka S et al. gone early, a novel germline factor, ensures the proper size of the stem cell precursor pool in the Drosophila ovary. PLoS One. 9, e113423 (2014)(PMID:25420147)
  2. Okazaki H et al. Calcineurin knockout mice show a selective loss of small spines. Neurosci Lett. 671, 99-102 (2018)(PMID:29427598)
  3. Urata Y et al. Spatio-temporal neural stem cell behavior leads to both perfect and imperfect structural brain regeneration in adult newts. Biol Open 7, bio033142 (2018)(PMID:29903864)
Product category
Tools
Epitope tags
Fluorescent proteins
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  • The availability is based on the information in Japan at 00:05, Jan 23, 2025 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.