Anti-ASC (TMS1) (Human) mAb

Availability (in Japan)

10 or more

（In Japan at 00:05,
Apr 19, 2024 in JST）

Size

100 µL (1 mg/mL)

Citations

Data Sheet

SDS

Data	Western Blotting Immunoprecipitation
Clonality	Monoclonal	Clone	23-4
Isotype (Immunized Animal)	Mouse IgG1
Applications	WB 1 µg/mL IP 5 µg/300 µL of cell extract from 5x10⁶ HL-60 cells IC* reported. （PMID: 10567338 / 15041718） IH* reported. （PMID: 11561011）
Immunogen (Antigen)	Triton X-100 insoluble fraction of retinoic acid-treated HL-60 cells
Reactivity [Gene ID]	Human[29108], Mouse(-), Rat(-)
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	PYCARD, TMS, CARD5, TMS-1
Background	ASC (apoptosis-associated speck-like protein containing a CARD (caspase recruitment domain)) is a 22 kDa soluble protein, located in the cytosol of HL-60 cells. In apoptotic HL-60 cells, it is able to be visualized as a speck, forming an insoluble aggregate. The C-terminal domain of this protein contains a CARD, suggesting that ASC may have proapoptotic activity in HL-60 cells. Recent data have indicated that ASC plays a role of adaptor protein linking various PAAD (Pyrin, AIM, ASC, and death domain-like) family proteins to pathways involved in NF-κB and procaspase-1 activation.
Related products	D086-A64 Anti-ASC (TMS1) (Human) mAb-Alexa Fluor™ 647
Citations	Masumoto J et al. ASC, a novel 22-kDa protein, aggregates during apoptosis of human promyelocytic leukemia HL-60 cells. J Biol Chem. 274, 33835-8 (1999)(PMID:10567338) Masumoto J et al. Murine ortholog of ASC, a CARD-containing protein, self-associates and exhibits restricted distribution in developing mouse embryos. Exp Cell Res. 262, 128-33 (2001)(PMID:11139337) Masumoto J et al. Pyrin N-terminal homology domain- and caspase recruitment domain-dependent oligomerization of ASC. Biochem Biophys Res Commun. 280, 652-5 (2001)(PMID:11162571) Western Blotting Mayor A et al. A crucial function of SGT1 and HSP90 in inflammasome activity links mammalian and plant innate immune responses. Nat Immunol. 8, 497-503. (2007)(PMID:17435760) Bryan NB et al. Differential splicing of the apoptosis-associated speck like protein containing a caspase recruitment domain (ASC) regulates inflammasomes. J Inflamm (Lond). 7, 23 (2010)(PMID:20482797) Immunocytochemistry Bryan NB et al. Differential splicing of the apoptosis-associated speck like protein containing a caspase recruitment domain (ASC) regulates inflammasomes. J Inflamm (Lond). 7, 23 (2010)(PMID:20482797) Terasawa K et al. Epigenetic inactivation of TMS1/ASC in ovarian cancer. Clin Cancer Res. 10, 2000-6 (2004)(PMID:15041718) Immunohistochemistry Masumoto J et al. Expression of apoptosis-associated speck-like protein containing a caspase recruitment domain, a pyrin N-terminal homology domain-containing protein, in normal human tissues. J Histochem Cytochem. 49, 1269-75 (2001)(PMID:11561011)
Product category	Research area Immunology Apoptosis

The availability is based on the information in Japan at 00:05, Apr 19, 2024 in JST.
The special price is shown in red color.
Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.