Anti-RCAS1 (Human) mAb

Availability (in Japan)

1-9

（In Japan at 00:05,
Apr 17, 2024 in JST）

Size

100 µL (1 mg/mL)

Citations

Data Sheet

SDS

Data	Immunohistochemistry
Clonality	Monoclonal	Clone	22-1-1
Isotype (Immunized Animal)	Mouse IgM
Applications	FCM 1-5 µg/mL (final concentration) IC 1-5 µg/mL IH* reported. （PMID: 15904507 / 17849467 / 24167369）
Immunogen (Antigen)	Human uterine cervical adenocarcinoma cells
Reactivity [Gene ID]	Human[9166]
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	EBAG9, EB9, PDAF
Background	RCAS1 (receptor-binding cancer antigen expressed on SiSo cells) is a novel tumor-associated antigen expressed in human uterine and ovarian carcinomas. The predicted amino acid sequence of RCAS1 (213 aa) possesses an N-terminal transmembrane region and a coiled-coil structure in the C-terminal portion, indicating that RCAS1 is a type II membrane protein able to form oligomers through the coiled-coil structure. RCAS1 revealed different expression pattern from the known tumor associated antigens such as, YH206, GA733, CA125, CEA and sialyl Lewis molecules in human tumor cell lines. Recent study indicated RCAS1 acts as a ligand for a putative receptor present on various human cells including T, B and NK cells. RCAS1 inhibited the in vitro growth of receptor-expressing cells and induced apoptosis. It was suggested that tumor cells might evade immune surveillance by expression of RCAS1.
Related products	D060-3H Anti-RCAS1 (Human) mAb M079-3 Mouse IgM (isotype control)
Citations	Flow Cytometry Wolf J et al. Role of EBAG9 protein in coat protein complex I-dependent glycoprotein maturation and secretion processes in tumor cells. FASEB J. 24, 4000-19 (2010)(PMID:20570965) Immunohistochemistry Reimer TA et al. Reevaluation of the 22-1-1 antibody and its putative antigen, EBAG9/RCAS1, as a tumor marker. BMC Cancer. 5, 47 (2005)(PMID:15904507) Theocharis S et al. RCAS1 expression in mobile tongue squamous cell carcinoma: an immunohistochemical study. Med Sci Monit. 17, BR228-234 (2011)(PMID:17849467) Giaginis C et al. Increased RCAS1 expression is associated with advanced histopathological stage and poor prognosis in patients with gastric adenocarcinoma. Dis Markers. 35, 213-9 (2013)(PMID:24167369) Giaginis C et al. Receptor-binding cancer antigen expressed on SiSo cells (RCAS1) expression in human benign and malignant thyroid lesions. Med Sci Monit. 18, BR123-9 (2012)(PMID:22460085) Leelawat K et al. Upregulation of tumour associated antigen RCAS1 is implicated in high stages of colorectal cancer. J Clin Pathol. 56, 764-8 (2003)(PMID:14514780) Oshikiri T et al. Tumor-associated antigen recognized by the 22-1-1 monoclonal antibody encourages colorectal cancer progression under the scanty CD8+ T cells. Clin Cancer Res 12, 411-6 (2006)(PMID:16428480)
Product category	Research area Cancer Apoptosis

The availability is based on the information in Japan at 00:05, Apr 17, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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