Anti-CD29 (Integrin β1) (Human) mAb-PE

Availability (in Japan)

1-9

（In Japan at 00:05,
Feb 20, 2019 in JST）

Size

1 mL (50 tests)

Citations

Data Sheet

SDS

Data	Flow Cytometry
Clonality	Monoclonal	Clone	AG89
Isotype (Immunized Animal)	Mouse IgG1
Applications	FCM 20 µL (Ready for use.)
Immunogen (Antigen)	G-361 (Human melanoma cells)
Reactivity [Gene ID]	Human[3688]
Storage buffer	PBS/1% BSA/0.09% NaN₃
Storage temp.	４°C	Conjugate	PE	Manufacturer	MBL
Alternative names	ITGB1, integrin beta 1, FNRB, MDF2, VLAB, GPIIA, MSK12, VLA-BETA
Background	The integrin family of adhesion molecules participate in important cell-cell　and celle-xtracellular matrix interactions in a diverse range of biological processes. Integrins are heterodimers consisting of a a subunit and b subunit. Both a and b subunit are transmembrane proteins with large extracellular domains (>100 kDa for a subunit and >75 kDa for b subunit) that interact with extracellular matrix proteins and relatively small cytoplasmic domains (50 amino acids or less, except for the b4 subunit) that interact with cytoskeletal proteins. The adhesiveness of integrins is dynamically regulated in response to cytoplasmic signals, termed “inside-out” signaling. It has been reported that, upon ligand binding, integrins regulate many intracellular signaling pathways that involve cytoplasmic alkalization, intracellular Ca²⁺ fluctuation, inositol lipid metabolism, protein kinase C, MAP kinase and phosphatidyl inositol kinase. Anti-integrin monoclonal antibody, AG89, reacts with human integrin b1 chain regardless of the a subunit. AG89 can recognize resting site b1 integrin on the cells, but the reactivity is increased ~2-fold upon integrin activation by anti-activating b1 antibodies and ~3-fold by Mn²⁺. Furthermore, occupation of the ligand-binding pocket by a soluble ligand (RGD peptide for a5b1 and CS-1 peptide for a4b1) resulted in maximum binding of AG89. The epitope for AG89 lies within residues 426-587.
Related products	D050-3 Anti-CD29 (Integrin β1) (Human) mAb M075-3 Mouse IgG1 (isotype control) W052-3 Anti-Osteopontin (SPP1) (Human) mAb D252-3 Anti-CD9 (Human) mAb K0046-3 Anti-Integrin α7 (Mouse) mAb
Citations	Flow Cytometry Matsunaga T et al. Potentiated activation of VLA-4 and VLA-5 accelerates proplatelet-like formation.　Ann Hematol. 91, 1633-43 (2012)(PMID:22644786)
Product category	Research area Stem cell research Cell surface antigens

The availability is based on the information in Japan at 00:05, Feb 20, 2019 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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