Blockmaster™ PA series
For
immunological
testsBlockmaster™CE510/CE210
Target Material
- Latex beads
- Magnetic beads (Material having a functional group capable of binding to the amino group)
- Colloidal gold particles
Mode of attachment
- Primary ･･･ Covalent coupling
- Secondary ･･･ Physical adsorption
-
For
immunological
testsBlockmaster™DB1130
Target Material
- Latex beads
- Magnetic beads
Mode of attachment
- Physical adsorption
Blocking agents
for multiple purposeBlockmaster™PA1080
Target Material
- Applicable to various materials and substrates (PSt, PP, COP, Glass, PDMS, PVDF, Nitro cellulose, etc)
Mode of attachment
- Physical adsorption
Fully synthetic physical adsorption type blocking agent
Blockmaster™ PA series is a physical adsorption type blocking agent which is fully chemical synthetic and can be used in a wide variety of applications. The unique water-soluble polymer with the proper balance of hydrophobic unit and hydrophilic unit proposed by JSR can prevent the adsorption of proteins or cells for microplate or petri dish made with different material.
Characteristics
- Virus/protein-free fully chemical synthetic blocking agent
- Can suppress the unspecific adsorption for various substrates
- Can be used in a wide variety of applications
- The quality of synthetic polymer is stable and can be assured
In the following table, the decreasing rate of protein (IgG) adsorption to various materials after Blockmaster™ or BSA treatment was calculated based on the one without any treatment. Blockmaster™ PA1080 shows very good performance in blocking for a wide variety of substrate material and effectively preventing the adsorption of protein.
| Polystyrene | Polypropylene | Cycloolefin polymer | Glass | Polydimethy lsiloxan | Polyvinylidene difluoride | Nitro cellulose | |
|---|---|---|---|---|---|---|---|
| Blockmaster™ PA1080 | ◎ | ◎ | ◎ | ◎ | ○ | ○ | ◎ |
| BSA | ◎ | ◎ | ◎ | ○ | △ | x | x |
Compared with untreated substratem the decreasing rate of protein (IgG) unspecific adsorption.
◎：over 90%, ○：over 80%, △：over 70%, x：less than 70%
Detail of product
| Grade | Package Volume | Solid Content | Solvent | Shelf Life |
|---|---|---|---|---|
| PA1080 | 100 mL | 1% | Water + 0.01% Proclin 950 | Labeled on the bottle |
| Grade | PA1080 |
| Package Volume | 100 mL |
| Solid Content | 1% |
| Solvent | Water + 0.01% Proclin 950 |
| Shelf Life | Labeled on the bottle |
※ There is also a 1000 mL package for raw materials for in vitro diagnostics. If you would like to purchase 1000 mL packaged product, please contact us.
Applications
Prevention of protein adsorption to various substrates
The amount of protein (IgG) adsorption was measured by adding the protein solution to polystyrene microplate which was treated with Blockmaster™ PA1080 or BSA and washed. The result shows that Blockmaster™ PA1080 can effectively prevent the adsorption of protein to the surface of polystyrene substrate.
Prevention of Cells adsorption
Using Blockmaster™ PA1080 for cell culture plate treatment can effectively prevent the adsorption of cell to the surface of substrate.
Effect of Blockmaster™ PA1080 on cells
The flollowing data shows that comparing with regular culture, the effect of PA1080 to cell survival rate and cytotoxicity. The results shows that even the addition amount was up to the 1% which is same with the product will not affect the survival rate or cytotoxicity.
There is no IC50* in the range of product concentration (10 mg/mL or less).* Half maximal inhibitory concentration
STORAGE
Please store at 2-8℃. Do not freeze the bottle.
