Blockmaster™ CE series

Covalent coupling type blocking agent

Blockmaster™ CE series, the unique water-soluble polymer proposed by JSR, has the end functional group that can covalently conjugate with the amino group on solid state. By suppressing the unspecific adsorption of protein, the ligands become easier to be activated, and therefore higher signal-to-noise-ratio can be achieved. It can use to replace BSA or use with BSA.

Characteristic

Polymer composition and functionality

The polyamine segment in polymer can be covalently coupled with the carboxyl group and anchored on the surface of material. On the other hand, the bearing cation can also promote the adsorption to the protein or surface material with negative charge via electrostatic interaction. Moreover, it can also be used as the dispersion stabilizer for the Colloidal gold with the cobvalent bonding of unshared electron pair.

THE MAIN COUPLING METHODS

The carboxyl groups on the surface of beads was activated to anchor the ligands including antibody. And Blockmaster™ CE510/CE210 was added to react with unreacted activated carboxyl groups to finish the blocking.

Detail of product

GradePackage VolumeSolid ContentSolventShelf Life
CE510, CE210100 mL2%Water + 0.01% Proclin 950Labeled on the bottle
GradeCE510, CE210
Package Volume100 mL
Solid Content2%
SolventWater + 0.01% Proclin 950
Shelf LifeLabeled on the bottle

CE510 and CE210 are different from the molecular weight of the main component polymer. (CE510: 5,000 (approx.) / CE210: 2,000 (approx.))

※ There is also a 1000 mL package for raw materials for in vitro diagnostics. If you would like to purchase 1000 mL packaged product, please contact us.

Applications

Prevention of protein adsorption / Signal enhancement

Using Blockmaster™ CE510/CE210 can promote the refolding of antibody and therefore the enhancement of signal can be expected since the activity of antibody is retained. Moreover the PEG segment in polymer can induce the exluded volume effect to achieve low noise.

Antibody/mixed-CE510/CE210 tethered chain co-immobilized
surface to improve immune response of the antibody

Dispersion stability

The dispersity of magnetic beads which are coated with Blockmaster™ or BSA was confirmed that the aggregation of beads can be prevented after Blockmaster treatment.

Magnetic beads dispersion

Colloidal gold particles dispersion

REFERENCES

STORAGE

Please store at 2-8℃. Do not freeze the bottle.

IMPORTANT NOTICE

Blockmaster™ series
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